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Thorough general knowledge of bioassay test methods (e.g. Elisa, Flow cytometry, qPCR, cell culture) Education: BA or MS in Cell biology Immunology, Molecular biology, virology, biochemistry, microbiology, or other related science.
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Experience in cell line development, ELISA, PBMC biomarker, cell culture, cellular assays, gene expression is desirable. Perform cell biology and molecular biology studies to support early drug discovery projects.
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Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation, and migration assays, Ca2 imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy.
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Manufacturing cGMP production activities include solution preparation, master/working cell bank production, upstream cell culture, downstream protein purification, mRNA and molecular therapeutics production, and small-scale fill/finish.
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Candidate must have strong technical skills in mammalian cell culture, and cell-based assay development using cell lines and primary cells. BS degree with 5+ years of relevant experience; or MS degree with 3+ years or PHD with 1 year of relevant experience within laboratory and industry, preferably in Cancer Biology, Cell Biology, Genetics, Pharmacology, Immunology, or a related scientific discipline.
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Direct experience in cell culture & molecular biology techniques highly preferred. The Department of Internal Medicine-Division of Rheumatology is hiring for a Research Assistant I/II. The ideal candidate for this role will have experience with cell culture: maintenance of cells using sterile techniques, harvesting cells to prepare for RNA or protein.
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Bachelor's or Master's degree in Biology, Molecular Biology, Biochemistry, or related field with 2+ years of industry experience in cell-based assay development. Experience with primary cell culture and flow cytometry is a plus.
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The PI is looking for a research technician with experience in molecular biology, cell culture, and laboratory mouse work, to participate in an NIH-funded project. A bachelor's degree in biology, biochemistry, or a related field plus working experience with cell lines, and/or primary culture.
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Mammalian cell culture, cytotoxicity analysis and multi-color fluorescence flow cytometry. Experience in primary gamma/delta T cell culture is desired. PhD in life science-related fields (Immunology, Molecular Biology, Pharmacology, Chemistry, and Biochemistry) or Master's with 6+ years in science-related fields.
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Skills Proficient in mammalian cell culture and general molecular biology techniques such as transient plasmid transfection, PCR, qPCR, ddPCR and Western blot. Education BS/MS degree in Cell Biology, Molecular Biology, Virology, Biochemistry, Genetics, or a related discipline with 3 years of laboratory research experience Thank you Deeksha Gupta Team Lead - Recruitment (1) 213-788-7669 Deeksha.
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Strong molecular biology expertise, with significant experience in mammalian cell culture and next-generation sequencing technologies. Perform multiple molecular and cellular biology techniques, including RNA transcription, tissue culture, cell transfection, qPCR, and other biochemical assays.
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Proficiency in tissue culture, cell engineering, and flow cytometry is preferred, experience with ELISA and cytotoxicity assays using Incucyte desired. The ideal candidate should have relevant experience in T cell immunology, cancer cell culture and flow cytometry, and enjoy fast-paced, collaborative, and vibrant startup culture.
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Prior laboratory experience, particularly with molecular biology techniques, including high-throughput assays and working with cell culture models. Knowledge of in vitro cell culture models, genetic modifications (siRNA, shRNA, CRISPR), DNA damage repair, immunoassays (multiplex ELISA) is preferred.
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Experience with molecular biology techniques, cell culture, qRT-PCR, genomics, proteomics and bioinformatics are preferred. Experience in Drosophila culture, molecular biology, microscopy Preferred Qualifications: Ph. D. in Biomedical Sciences and record of significant participation in research activities as evidenced by peer-reviewed publications.
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Knowledge of research techniques or methods (cell biology, translation, transcription, primary cultures, microscopy, cell assays, biochemical assays, molecular biology assays.
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