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Strong knowledge and hands-on experience in cell biology and biochemical assays such as cell culture, ELISA and Western-blot. Master’s degree in molecular and cell biology, biochemistry, biotechnology, or other relevant biological sciences with at least 1 years’ experience or Bachelor’s Degree with at least 3 years of experience.
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Experience in mammalian and bacterial cell culture, bioreactors, protein purification, plasmid DNA purification, tangential flow filtration and various other filtration techniques is preferred.
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Additional skills of interest include experience in one or more of the following areas: biochemistry, protein extraction, metabolite/lipid extraction, cell culture, proteomics, metabolomics, or lipidomics, and/or microbiology.
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The department also houses a vivarium for animal husbandry, associated animal lab testing areas, cell culture capabilities, fluorescence microscopy, qRT-PCR machine, and Cryostat to perform cell/molecular work.
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The incumbent will carry out cell culture/bioengineering experiments supporting the key research projects in the lab including development/applications of the Next-Generation Lung-on-a-Chip and ECM MV-Chip technologies as well as various aspects of the Down syndrome pathophysiological modeling including using Vascular-on-Chip microfluidic device.
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Familiar with DNA Isolation and Purification, PCR, Cell Culture, Sequencing, etc. BS/BA in a biological science/biomedical engineering, similar field of study, or comparable industry experience.
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Technical skills and competencies to conduct quantifiable, meaningful research using cellular and molecular biology techniques, such as flow cytometry, ELISA/ELISPOT, tissue processing, cell, bacterial, viral and/or parasite culture, sterile technique, DNA/RNA extractions, PCR.
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Hands-on experience and in-depth knowledge of cellular and molecular biology techniques, at least one area of 1) cell/tissue culture, 2) gene expression analysis such as qPCR, and 3) protein analysis such as Western blotting and ELISA.
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The laboratory utilizes genetically engineered mouse models, primary cell culture, retroviral gene transfer technologies, protein purification, genomics, microscopy, computational biology, and patient sample analysis, to answer biological questions related to tumor initiation and survival.
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Integrate diverse data sources into model development and optimization (in vitro cell culture, immunoassays, clinical studies, large-scale omics data, etc) 3+ years of experience developing computational models of biological systems and simulations in drug discovery/development.
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Successful candidates will have a degree in Chemical Engineering, Biological Science or related discipline and preferably have experience with some of the techniques utilized for either upstream (e.g. mammalian cell culture in controlled bioreactor systems) or downstream (e.g. liquid chromatography methods) manufacturing processes.
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Individuals with a scientific background, mammalian cell culture and cGMP experience needed. Conducting routine manufacturing of biologic products including media preparation, cell culture, cell counting, formulation, centrifugation and freezing.
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Preparing diagnostic reagents which include bacterial culture, histology preparations, entomology preparation, inoculation of cell cultures, animals, and embryonating chicken eggs; as well as collection of animal specimens.
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Mammalian cell culture and molecular biology skills. Bachelors degree in a relevant Biological Science discipline is required. Prepare and maintain mammalian cell cultures. Experience with laboratory methods including qPCR, flow cytometry, RNA/DNA isolation, Western Blot, ELISA, etc.
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BS or MSc in Biological or Biochemical sciences, and 3-5 years relevant laboratory experience in antibody/protein characterization or quality control – particularly in the areas of immunology, molecular and cell biology, and protein chemistry.
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