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Skills & Abilities: Extensive scientific HLA knowledge and technical skills in the following areas preferred: HLA serology and molecular genetics, HLA antibody screening /identification of HLA antibody specificity, cross matching techniques to support stem cell transplantation programs and platelet transfusion therapy and other areas in the field of Histocompatibility and Immunogenetics.
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Support antibody expression construct generation by molecular cloning, DNA sequencing, and plasmid DNA purification. Antibody characterization/QC by SDS-PAGE, HPLC-SEC, and Gator to determine antibody quantity and quality.
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Mosaic teams provide comprehensive, end-to-end discovery services, including program assessment and strategy development; program management; antibody discovery and optimization; protein production, purification, and characterization; protein engineering; biochemical and cellular assay development; formulation, stability and developability studies; bioanalytical (PK and PD) assay development; and candidate-validating pharmacology.
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Avidity Biosciences, Inc.'s mission is to profoundly improve people's lives by delivering a new class of RNA therapeutics - Antibody Oligonucleotide Conjugates (AOCs™). Avidity Biosciences, Inc.'s mission is to profoundly improve people's lives by delivering a new class of RNA therapeutics - Antibody Oligonucleotide Conjugates (AOCs.
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We are currently seeking a Research Associate II/Senior Research Associate to join our Protein Engineering and Structural Biology Team. You will play a significant role in performing broad antibody characterization to support our reverse immunology platform.
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Familiarity with DNA, RNA, enzyme, antibody, and antigen chemistry is highly desirable. Our team engages in a wide range of activities, from cutting-edge organic synthesis to innovative formulations chemistry, developing novel coatings, and supporting an array of other programs.
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Has hands on experience with executing antibody or antibody-like molecule purification techniques including resin chromatography (affinity, CEX, AEX, MMC), depth filtration, virus filtration, membrane chromatography, and TFF operations.
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As an Advanced Research Associate you will work in the Antibody Validation Lab assisting with the validation of antibodies for Immunohistochemistry, Spatial Biology, and Western Blot. As a member of the Antibody Validation lab, you will use high-content antibody characterization methods to generate comprehensive datasets detailing the binding of antibodies to their antigen targets.
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Broad familiarity of difference disciplines within the life sciences ( e.g. PCR, recombinant DNA technology, protein expression and purification, chromatography, immunology, and antibody chemistry and binding kinetics.
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This will include formulation and commercial process development for biologics (monoclonal antibodies, antibody drug conjugates, bispecifics and fusion proteins). The Biologics Drug Product Development group develops monoclonal antibodies, antibody drug conjugates (ADC), and other biologics using state of the art formulation and process technologies.
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Operation of antibody-based immunogenicity assays (neutralizing antibody assay and total binding antibody assay) Tissue Culture work: maintenance of in vitro and ex vivo (primary) cell culture.
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At Genmab, we’re committed to building extra(not)ordinary futures together, by developing antibody products and pioneering, knock-your-socks-off therapies that change the lives of patients and the future of cancer treatment and serious diseases.
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Perform expression and purification of mono- and multi-specific antibodies and complex modalities fusion proteins from sub-milligram to gram scale to support antibody discovery, characterization, and pre-clinical validation of leads.
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Ideal candidate will have a significant background in plate–based assay development and antibody characterization including HPLC and Octet. BSc/MSc 3–6 + years in Molecular Biology, Chemistry, Analytical Chemistry, Organic Biochemistry considered, or equivalent combination of education and experience.
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ResponsibilitiesExtract protein, DNA and/or RNA from necropsied animal tissueRun ddPCR, qPCR, ELISA, Western Blot etc analysis on tissue extractsCell culture, Neutralizing antibody assays and total antibody assaysSample analysis assay optimizationSOP writingData recording and analysisQC analysis This position directly impacts preclinical outcomes including proof of concept, ED50, MED, clinical hold response and IND enabling studies.
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