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Previous hands-on experience on flow cytometry assays, DNA/RNA isolation, PCR, gene cloning, single cell technology, mammalian cell. Design, plan and execute basic to complex cell-based and molecular biology experiments including tissue culture, flow cytometry analysis, cytokine production and cell activation assays.
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Advanced knowledge of molecular biology techniques such as cloning, site-directed mutagenesis, DNA isolation and purification, RT-PCR, and qPCR.Experience working with RNA and/or DNA viruses and reverse genetics systems.
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In-depth knowledge and hands-on expertise in molecular biology, including but not limited to qPCR, ddPCR, DNA and RNA isolation, advanced molecular cloning techniques, molecular probe techniques, and sequence modifications.
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QPCR using the Life Technologies QS5 systemMolecular Biology DNA, RNA, and protein purification and quantification, gel electrophoresis, molecular cloning, site-directed mutagenesis, and recombinant protein expression.
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Maintain accurate, daily records of experiments and results in pen in bound laboratory notebooks and perform procedures such as molecular techniques, RNA/DNA extraction, RT-PCR, Real Time PCR, immunostaining, flow cytometry, maintaining cell cultures, ELISAs, Western blot, preparation and analysis of RNA/DNA sequencing, in vivo inoculations, necropsy and tissue collection/processing; experimental design, and basic statistical analysis.
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Prepare DNA, RNA and/or protein samples, including libraries and PCR. Prepare DNA, RNA and/or protein samples, including libraries and PCR. This position is best suited for individuals who are looking to work long-term (2 - 3 years or more) on a government contract, previously have experienced managing hands-on laboratories, and have experience working with animals, specifically rodents.
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DNA enzymology especially DNA and RNA polymerases. This is a full time position, and the title can be Senior Scientist or Principal Scientist. Experience developing molecular diagnostics, point-of-care nucleic acid tests, and isothermal amplification methods.
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Carry out molecular biological experiments, including cell culture, tissue homogenization, protein and RNA extraction techniques, multiplex enzyme-linker immunosorbent assay, Western blotting, RT-PCR, tissue handling and flow cytometry.
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Technical hands-on knowledge of basic molecular and cell biology techniques, such as PCR (qPCR/dPCR), DNA/RNA isolation and characterization (UV/Vis spectroscopy, gel electrophoresis, other analytical methods), and basic mammalian cell culture techniques strongly preferred.
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Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR. Rescue and characterize pseudoviruses and/or RNA viruses.
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Understanding of DNA sequencing techniques (NGS and Sanger) and sequence analysis. A background in molecular/clinical virology and/or vaccinology is required. Assist in the preparation and review of bioanalytical data and reports suitable for regulatory submission.
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Prior experience with RSV, HNPV or other RNA respiratory viruses. Requirements:Bachelor 's Degree in virology, molecular biology, immunology, or another related field with 2+ years of relevant industrial (preferred) or academic experience or a Master's Degree with some relevant.
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Apply molecular biology skills to generate recombinant proteins and viruses. Understanding of molecular cloning techniques and vector engineering. The work will be focused heavily upon molecular viral surveillance and resistance monitoring for the CLIENT infection franchise.
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The contractor shall: Perform bioinformatic support using standard computer software packages for data management including data checking and cleaning of genotype and phenotype data, merging data, creating and recoding variables to prepare data for analyses, analyzing omics (DNA and RNA) data using a variety of standard statistical techniques including classical regression and statistical genetics tools.
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Experience in molecular biology and genetics/genomics including DNA/RNA isolation and manipulation, NGS library prep, QC, and sequencing is preferred. Perform experimental procedures using molecular biology techniques such as isolation of DNA/RNA, Quantification, NGS library construction, qPCR, operating sequencers, micro pipetting, and meticulous measuring.
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rna job in Rockville, MD
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