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Experience with protein expression, purification, and characterization using analytical methods such as SDS-PAGE, Western blot, HPLC, and ELISA. Seismic Therapeutic, a biotechnology startup focused on the intersection of structure, protein engineering, machine learning and immunology, is seeking an experienced and motivated Associate Scientist to help drive novel immunotherapy programs.
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Expertise in various analytical techniques for protein characterization (e.g., SDS-PAGE, ELISA, Western blot, protein quantitation, FTIR, DSC, particle analysis). PhD (preferred) in biological sciences, biochemistry, or bioengineering, with a specialization in protein biochemistry.
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Operate LCMS, ELISA, PCR, GCMS, and ICP-MS equipment to analyze pre-clinical samples provided by formulation teams. Operate LCMS, ELISA, PCR, GCMS, and ICP-MS equipment to analyze pre-clinical samples provided by formulation teams.
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Strong laboratory background with proficiency in several biological techniques (ELISA, ECL, mass spectrometry, PCR, etc.) As a Principal Scientist in the Translational Medicine Drug Disposition Bioanalytical group, you will have the opportunity to make a difference.
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Proficiency in mammalian cell culture techniques, flow cytometry, qPCR, DNA and RNA extraction, transfection, ELISA, and western blotting. The current Flagship ecosystem comprises 41 transformative companies, including Axcella Health (NASDAQ: AXLA), Denali Therapeutics (NASDAQ: DNLI), Evelo Biosciences (NASDAQ: EVLO), Foghorn Therapeutics (NASDAQ: FHTX), Indigo Ag, Kaleido Biosciences (NASDAQ: KLDO), Moderna (NASDAQ: MRNA), Rubius Therapeutics (NASDAQ: RUBY), Sana Biotechnology, Seres Therapeutics (NASDAQ: MCRB), and Sigilon Therapeutics (NASDAQ: SGTX.
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With analytical techniques, such as QPCR, PCR, SDS-PAGE, Western blotting and ELISA - Exp. with electronic laboratory notebook applications, such as Benchling - Basic knowledge and application of theories, principles and techniques in molecular biology and cell biology "Nice to Haves": - Experience in a QC/GMP testing environment a plus Duties/Responsibilities: - Operate and maintain standard laboratory equipment and department-specific equipment.
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The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Experience in analytical techniques including ddPCR, ELISA, and mammalian cell culture. Utilize analytical methods such as ddPCR, cell-based methods, ELISA, capillary electrophoresis (SCIEX), as well as standard compendial methods.
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Other analytical techniques such as ELISA, HPLC, ELISPOT assay. In our Brighton/Boston labs, RNA biology meets today's cutting-edge genomics, computational biology, and deep-sequencing technologies to create a new class of medicines that address the underlying causes of disease.
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Perform advanced procedures such as immunohistochemistry assay, apoptosis studies, flow cytometry, genotyping, qPCR, RTpCR, Western Blots, ELISA as required to evaluate nanoparticle efficacy. Experience with cell culture techniques, molecular biology techniques (western blots, ELISA etc) and fluorescence imaging is a must.
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Perform routine/non-routine testing of in-process, DSI, final product, and raw material samples in accordance with Standard Operating Procedures, such as Visual Appearance Inspection, Flow Cytometry using BD FACS Canto, ID tests by FTIR, pH/Conductivity, Osmolality, Cell Count and Viability by Cellometer, Nucleic Acid concentration by NanoDrop, ELISA by Plate Reader, Colony count by StemVision, sickling reduction by ImageStream Analysis, HPLC/UHPLC, qPCR/ddPCR, and other applicable test methods.
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Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq. Broad technical expertise/knowledge including with flow cytometry, ELISA, immunohistochemistry, qRT-PCR, NGS and RNA Seq.
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Running ELISA assays on human blood and urine samples. This person will focus on the implementation and analysis of individual research projects that will include measuring biomarkers using ELISA assays and proteomic platforms.
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Erformance of routine biological assays including qPCR, ddPCR, ELISA, SDS-PAGE, and agarose gel electrophoresis is. Candidates should be self-starters, comfortable with autonomy, and embrace opportunities to develop new genomic medicines as part of a dynamic team within Alexion.
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