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Primary responsibilities include but are not limited to designing and performing high-throughput antibody characterization assays using SPR and BLI technologies (required) as well as other immunoassay and bioanalytical platforms such as ELISA and FACS (nice to have.
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The teacher must be confident and comfortable in a wet lab space teaching students about the basics of laboratory research and current laboratory techniques such as gel electrophoresis, DNA extraction, PCR, and ELISA. In addition, the teacher will be responsible for ordering reagents and materials, preparing experimental protocols, and setting up the equipment for each session.
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Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc. Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc.
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Other relevant experience includes multianalyte analysis from media or cell lysates (e.g. Luminex), ELISA, DNA and RNA isolation, PCR and RT-PCR, gene editing, various microarray technologies. Experience with FACS-based ELISA methods such as LEGENDplex assay and softwareExperience with Adobe Photoshop & IllustratorAt Dana-Farber Cancer Institute, we work every day to create an innovative, caring, and inclusive environment where every patient, family, and staff member feels they belong.
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The ideal candidate should possess strong laboratory experience and technical expertise in in vitro cellular immunology (including ELISA, MSD, Co-IP, assay development, tissue culture, flow cytometry, primary immune cell assays, cell fractionation) and ex vivo tissue processing and analysis.
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Proven hands-on experience with cell-based and molecular biology assays such as: cell viability/cytotoxicity, flow cytometry, mRNA expression analysis (qPCR/ddPCR/RNseq), western blotting, ELISA among others.
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Principal duties and responsibilities will include performing cutting edge, bench-level research in the following areas: molecular/cellular biology, human induced pluripotent stem cell (iPSCs) and neural stem cells, cellular differentiation, lineage phenotyping and basic neurobiology.
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Point person for conducting key analytical testing (FACS, IHC, ELISA) of differentiated and edited cells. To accelerate and expand its efforts, CRISPR Therapeutics has established strategic partnerships with leading companies including Bayer and Vertex Pharmaceuticals.
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Provide continuous technical guidance to customers on experimental design pre- and post-instrument sale, including guidance on instrument usage, the impact of different processes/reagents on platform performance, and post-transfection readouts (flow cytometry, ELISA, PCR.
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Students involved in this project will be exposed to basic and advanced immunologic techniques such as FACS-sorting of immune cells, high-dimensional immunophenotyping of monocytes by multi-color flow cytometry, cellular assays, real-time PCR, ELISA, immunofluorescence microscopy, as well as transcriptional profiling of cells using bulk RNA and single-cell RNsequencing.
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Familiarity with technologies commonly used to assess immunological endpoints, including ELISA/MSD, western blot/JESS and multi-parameter flow cytometry. Familiarity with technologies commonly used to assess immunological endpoints, including ELISA/MSD, western blot/JESS and multi-parameter flow cytometry.
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Hands-on experience and ability to work with minimum supervision with standard molecular biology techniques (PCR, cloning etc), ELISA, MSD, Incucyte and other standard cell based, and non-cell based biochemical assays is required.
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The successful candidate will be responsible for generating preclinical data to support development of various compounds, via the execution of preclinical in vitro and supporting in vivo studies, to screen, profile and strengthen our mechanistic biological understanding of drug molecules.
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Experience with immuno-pharmacological techniques including in vitro and ex-vivo immune cell-based assays, ELISA, MSD, flow cytometry based immune cell profiling. Knowledge of biomarker discovery and analysis is highly desirable while experience with in vivo pharmacology is preferable.
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Experience with standard immunological assays such as flow cytometry / FACS, magnetic cell sorting, ELISA/Luminex, immunofluorescence staining and confocal microscopy, cell proliferation assays, cytotoxicity assays.
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