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Job Description: BioMedicine Design (BMD) is seeking a highly motivated Biochemist/Molecular Biologist to join Biotherapeutic Expression and Purification group (BEP) in Cambridge, MA. Qualified candidate will play an essential role in a high-energy DNA production and protein expression research laboratory.
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The BEP DNA production and protein expression group is looking for a laboratory technician to provide DNA production support for the protein expression and purification activities in BEP. The candidate is expected to have a strong background in molecular biology techniques and experience with nucleic acid production and analysis.
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A productive impact on the development of exosome purification from different biofluid samples for different downstream products: RNA, DNA, TNA, protein, whole exosome. Proficient in knowledge of molecular biology tools such as primer probe design tools such as VisualOMP, qPCR, dPCR, open array, nanostring, sample prep platforms of automated benchtop or liquid handling formats, various commercial sample prep kits, robust assay development methodology such as design for six sigma; hands-on experience is a plus.
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Downstream AKTA Protein Purification & Chromatography experience in GMP environment. Develop and optimize downstream processes for the purification of therapeutic proteins, ensuring high yield, purity, and product quality.
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We utilize the latest software and automation to develop low, mid, and high throughput methods for protein and nucleic acid purification, enzyme kinetics and biophysical characterization.
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Additional skills to support downstream characterization as needed would be beneficial and could include: protein expression and purification using FPLC systems and biophysical characterization methods (SEC-MALS, DSF, and biosensor techniques including BLI and SPR.
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Perform small-scale purification process including ultracentrifugation, column chromatography, cell lysis, clarification, AKTA and TFF unit operations, and depth filtration for manufacturing of LVV gene therapy vectors across multiple preclinical programs and disease areas to ensure on time delivery.
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Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations. The candidate will be a key contributor to the scientific team responsible for protein expression and purification.
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Experience with protein purification, filtration, and analytical characterization. Ensure development of phase-appropriate drug substance processes for portfolio of bispecific antibodies and cytokine fusion molecules, including cell banking, upstream cell culture, and downstream purification.
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Expertise in biologics Purification development such as nucleic acids protein, and/or AAV. Sail Biomedicines is seeking a highly motivated and innovative Associate Director, Drug Substance Process Development to be the subject matter expert and leader of RNA manufacturing processes, including upstream and downstream activities.
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Must have 3 years of post-bachelor working experience in a Biotech, Pharma, or Academic lab with hands-on experience in protein purification using lab scale AKTA instruments, UNICORN software, and chromatographic resins.
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The group supports non-GMP transient protein lead production across the companies diverse biotherapeutic discovery portfolio to enable the research units and their pre-clinical therapeutic in-vitro and in-vivo needs.
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10+ years experience in biologics process development and a strong working knowledge of antibody processing, including upstream and downstream processes. Lead and mentor scientists/engineers with upstream and downstream processing expertise by fostering an environment for career growth.
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Perform small-scale bioprocess experiments that encompass both upstream (cell culture) and downstream (purification) operations. Purification experiments include, but are not limited to, protein chromatography, filtration, and UF/DF operations.
$75,623 - $92,226 a yearExpandUpdated Today
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