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Proven expertise in membrane protein purification by affinity chromatography, SEC, IEX using AKTA systems. The successful candidate will perform laboratory research including membrane protein purification, structure determination, biophysical/biochemical characterization and cell-based assays using state-of-the-art resources.
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The Susa lab is starting the Department of Pharmaceutical Chemistry at UCSF and is seeking applicants for a research technician position using structural biology, membrane protein biochemistry, cell biology, and protein engineering to understand fundamental mechanisms of transmembrane signaling within the human immune system.
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Experience with large molecule characterization by LCMS (Intact protein MS and bottom-up proteomics experience required; experience with intact oligonucleotide analysis a plus) Strong understanding of relevant concepts in cancer biology, protein biochemistry, protein structure, and a general understanding of chemical reactivity is required.
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Primary Responsibilities: Primary contributor with protein characterizing mass spectrometry experiments, including maintaining the instrument, developing and performing experiments with an emphasis on intact mass, glycan analysis and peptide mapping studies.
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Hands-on experience using HPLC, SDS-PAGE, mass spectrometry as applied for protein analytical method development. Hands-on experience using HPLC, SDS-PAGE, mass spectrometry as applied for protein analytical method development.
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Nobell is seeking a Computational Biologist / Machine Learning Scientist to join our team of scientists to contribute to the development of the Nobell Plant Grown Protein (PGP) platform. Develop and implement algorithms across a range of in-house acquired data including, but not limited to genetic sequences, RNA expression, protein expression and protein structure.
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Demonstrated experience with routine molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence) + Analyze gene and protein expression through the application of a variety of techniques (RT-PCR, qPCR, digital PCR, western blot, immunofluorescence, and ELISA.
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The candidate will utilize molecular, cellular and computational biology, along with state-of-the-art single-cell technologies, to study translational control and protein homeostasis in the context of drug resistance.
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Broad experience and expertise in molecular reagent generation and cell line engineering using gene editing approaches (CRISPR) as well as development and implementation of novel cellular target engagement, activity, and PPI assays (e.g., immunoassays, second messenger generation, ion flux, reporter, protein stability, PPI (splitNanoLuc, splitβ-gal), gene activation, cellular imaging.
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Skills: Molecular Biology, Cell Biology, Biology, Chemistry, Chromatography, Protein Purification, Cell Cultures, Manufacturing Operations. Experience in cell culture and/or protein purification.
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Critically evaluate computational and wetlab assay methods in publications used as evidence for locational protein targets. Develop novel computational methods as needed to advance our understanding of interactions between EVs and locational protein targets.
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Junior or Assistant Specialist (Protein Biochemistry) - Kortemme Lab. One year or more of research experience in protein biochemistry or high-throughput technology in academia or industry.
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Reporting to the Pilot Plant Manager, the Pilot Plant Associate will participate in upstream and downstream areas of AAV development in the Pilot Plant for new gene therapy programs and processes. Display understanding of upstream and downstream process theory (i.e., Fermentation, Harvest, Tangential Flow Filtration, Column Chromatography) and equipment operation.
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Knowledge of cell biology, cell engineering, molecular biology, recombinant protein purification and protein characterization is preferred. RMG, in the Cell Culture and Bioprocess Operations department, is responsible for providing support to pre-Early Stage Research to Early Development projects with high-throughput protein production from mammalian transient cultures.
$35.67 - $52.44ExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Molecular biology techniques: perform molecular biology techniques such as PCR, RT-qPCR, and western blotting to analyze gene expression and protein levels. We are looking for a research associate whose primary role will focus on culturing induced pluripotent stem cell (iPSC) and performing molecular biology techniques to understand gene expression regulation during in vitro cardiac differentiation.
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protein job in San Francisco, CA
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