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Perform laboratory studies using complex techniques including large-scale tissue culture (maintaining mammalian cells, growth assays, drug response assays, transfection and viral infection), nucleic acid extraction, PCR analyses, immunoblotting, immunocytochemistry and immunohistochemistry.
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The Tissue Culture Specialist leads the scientific and production aspect of the company's tissue culture program which is responsible for preserving genetics and restoring diseased cannabis cultivars when possible, with various available methods.
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The research assistant will be responsible for performing tissue culture and basic molecular and biochemical lab techniques (PCR, immunoprecipitations, western blots) and will have the opportunity to learn early drug development, proteomics, and genomic screening approaches.
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These may require basic blood separation, labeling and assay methods such as ELISA, immunohistochemistry, flow cytometry, MACs bead isolations and other associated specialized laboratory methods including human iPSC tissue culture and molecular biology techniques.
$15 - $20.4 depends on experienceFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Effective management and maintenance of the tissue culture facility to uphold sterile conditions. Regular restocking of laboratory spaces, communal areas, and assistance with tissue culture requirements.
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Experience in recombinant cell line development, primary cell line isolation, tissue digestion. Proven mammalian cell culture and bioassay experience with recombinant and primary cells. Maintains order in cell culture and analytical laboratory, and adheres to cleanroom guidelines and aseptic technique, according to protocol.
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Upon demonstrated ability, the candidate will then have opportunity to learn one or more advanced imaging techniques (e.g. light sheet microscopy, single molecule localization microscopy, structured illumination microscopy, multiphoton in vivo imaging) or sample preparation methods (e.g. tissue clearing, expansion microscopy, correlative light and electron microscopy.
$105,040 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Other duties may include: Tissue culture, preparation of microbial cultures, ELISA, Western blotting, quantitative RT-PCR, flow cytometry, immunohistochemical and quantitative analysis of tissue samples, organization and maintenance of samples and other laboratory stock material.
$36.14 an hourFull-timeExpandApply NowActive JobUpdated 12 days ago - UpvoteDownvoteShare Job
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Experience in and/or development of abilities to use equipment items to perform PCR , Western blotting, tissue sections, immunohistochemistry, immunofluorescence, cell culture, flow cytometry, optical coherence tomography, ultracentrifuge, and others.
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Expertise in primary human cell culture, multi-color flow cytometry, multiplex cytokine detection, functional immune cell assays (proliferation, cytotoxicity, chemotaxis) and assay development is essential.
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Strong laboratory experience and technical expertise in cellular immunology techniques (e.g., ELISA, assay development, tissue culture, flow cytometry, primary immune cell assays, and ex vivo analysis.
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Equipment Utilized Tissue culture hoods and incubators, centrifuges, electrophoresis apparatus for proteins and nucleic acids, electroporator, gentleMACS Octo Dissociator, flow cytometry, multimode plate reader, thermal cyclers, nanodrop, light, confocal, and fluorescent microscopes, histopathology processor, embedding center, microtome, cryostat, and vibratome, ELISA plate washer, ELISA plate reader, ddPCR, qPCR, and other equipment as needed.
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A general knowledge of immunology and immune-oncology field a well experience in tissue culture and cell-based assays and flow cytometry is a plus. We have an inclusive and diverse culture that provides comprehensive benefits, which often include (depending on location) health and wellness programs, fitness centers, equity awards, annual bonuses, and paid time off for eligible employees at all levels.
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What We Offer Our Massage Therapists: Our Culture - At the Yonkers Ridge Hill Hand and Stone Massage and Facial Spa, we value diversity, inclusivity, and career longevity. Knowledge of other various modalities preferred (Neuromuscular, Myofascial, Trigger Point, Pre-Natal, Craniosacral, Reiki, Deep Tissue), but not required.
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Preferred Education, Experience and Skills: Proven experience in the following scientific techniques and procedures: tissue/cell culture; protein extraction; RNA isolation, ELISA, Western blot analysis, qRT-PCR. Proven experience with Mac and PC with intermediate knowledge using Workday; Microsoft Word, Outlook, Excel, and PowerPoint; Safari/Firefox; and Zoom. Weekend Hours Required.
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