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Experience with flow cytometry, functional assays, DNA, RNA and protein analysis. Cell culture and molecular biology skills are required. Sterile tissue culture. RNA/DNA isolation from cells or tissues.
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This role encompasses diverse responsibilities, including tissue culture, RNA and protein analysis, and hands-on work with laboratory mice. Position Overview: As a Laboratory Technician specializing in tissue culture and animal work, you will be at the forefront of our ambitious project to develop a stem cell therapy for Sanfilippo syndrome, a rare disorder.
$45,000 - $55,000 a yearExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Other technical responsibilities may include bacterial and mammalian cell culture (including organoid cultures), ELISA, and immunoblotting, molecular biology techniques such as cloning, RNA/DNA extractions, PCR and single-cell PCRs and imaging techniques including brightfield, confocal, and 2-photon intravital imaging.
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Innovate workflows for rapid DNA/RNA extraction, next generation sequencing (NGS) and bioinformatic analytics to include engineering detection. A strong understanding of molecular biology laboratory techniques including: cell and microorganism culture, sterile technique, cloning, PCR/qPCR, NGS library preparations and NGS sequencing platforms.
$50,900 - $89,100 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Expertise in molecular biology (PCR, RT-PCR, RNA seq), cell culture, immune functional assays utilizing patient samples, ELISA assays, and flow cytometry (FACS). Expertise in molecular biology (PCR, RT-PCR, RNA seq), cell culture, immune functional assays utilizing patient samples, ELISA assays, and flow cytometry (FACS.
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Familiar with basic assays such as tissue culture, cell lines and tissue, analyzing protein and RNA signaling assays (western blot, qPCR, etc.) UPMC Magee-Womens Hospital is seeking a Full Time Senior Research Assistant, BS to support the Gynecological Oncology lab.
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Preferred:Experience working with DNA, RNA, cell culture, qRT-PCS, and single-cell RNseq and proteomics. Perform molecular and cellular techniques: sample preparation and storage, ELISA, immunofluorescent staining, cell culture, PCR, gel electrophoresis 3 data analysis and documentation.
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The Department of Internal Medicine-Division of Rheumatology is hiring for a Research Assistant I/II. The ideal candidate for this role will have experience with cell culture: maintenance of cells using sterile techniques, harvesting cells to prepare for RNA or protein.
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Assist with ongoing projects in the laboratory which may involve performing the following activities: cloning, plasmid preps, PCR genotyping analysis, DNA and RNA extraction and purification, preparing samples for sequencing experiments, tissue culture, culturing of fungi and bacteria, microscopy, bioinformatics and/or data analysis.
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The qualified candidate will utilize systems immunology techniques including flow cytometry, cell culture, bacterial culture, single cell RNA sequencing, cytokine analysis, cell isolation, and data analysis.
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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry) transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA.
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The incumbent will perform tibial fractures of mice with various genotypes, evaluate the fractured bones at different times following fracture using methods including histomorphometry, immunohistochemistry, quantitative computed tomography, lineage tracing, cell culture, and genome analysis analyzing the effect of abaloparatide on these processes.
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Primary duties include assisting with daily laboratory experiments involving techniques on biochemistry, molecular biology, cell culture, and histology. An ideal candidate would have knowledge of basic molecular techniques, including DNA, RNA and protein western blot, polymerase chain reaction and the ability to independently prepare or supervise preparation of samples for analysis or testing after laboratory training.
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Preferably experience with animal models of disease, gene expression analysis, cell culture, gene transfection, viral gene transfer, tissue level analysis (histology, IHC). Discovery is pursued by the application of multiple Omics technologies (bulk RNsequencing, single cell RNA sequencing and spatial transcriptomics.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
$57 - $60 an hourExpandApply NowActive JobUpdated 1 month ago
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