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Sample accessioning using LIMS; The position requires a strong background in cell biology, particularly single cell NGS / Omics workflows. SeqMatic has a Research Associate / Associate Scientist position open to join our single cell sequencing services teams.
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Duties include but are not limited to:Independently manage a project in the laboratory and design experiments using molecular biology technical procedures including sample processing, functionalization and characterization of solid supports with nucleic acids and/or peptides, PCR, capillary gel electrophoresis, HPLC, plate readers, NGS library preparation, bioconjugation strategies and biomolecule purification techniques.
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Developing and optimizing protocols for NGS based DNA and RNA analytics and for ddPCR. Conduct sample and library preparation for RNA sequencing, besides using high-throughput methods for DNA and RNA extraction.
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This includes development of and overseeing the NGS workflow (sample management, library preparation, sequencing, and basic data analysis). In this role, the candidate will work collaboratively and cross-functionally with colleagues to develop molecular methods (PCR) and Next-Generation Sequencing (NGS) workflows that support the surveillance, diagnostics, and research efforts of the California Department of Public Health.
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The Molecular Technologist II (Sun - Thurs 9:00am - 5:30pm) is responsible for performing high complexity laboratory testing on patient specimens, to include the isolation of nucleic acids and next generation sequencing (NGS) with all associated procedures.
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Sample Management : Establish and manage a biomarker sample storage and inventory plan, including identifying third-party vendors, ensuring regulatory compliance, and developing a sample database and tracking system in collaboration with biology, clinical science, and bioinformatics teams.
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O Sample prep for the extraction of biomolecules such as nucleic acids (DNA and RNA) and protein, downstream processing and analysis, using Covaris’ proprietary AFA™ technology. o Analysis of nucleic acids, such as quantification using various methods, including absorption, fluorescent quantification, qPCR and digital PCR mediated assessment of quantities and quality, electrophoretic molecular weight analysis, NGS library construction.
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Integrate appropriate clinical data with Next-Generation Sequencing (NGS) output. The primary functions rely on leveraging FoundationCORE, FMI’s 300,000+ tumor sample dataset, to investigate novel cancer gene alterations (including annotation using publicly-available online resources such as UniProt and The Cancer Genome Atlas Program) and prepare scientific manuscripts.
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Expertise, in-depth hands-on experience is expected in genomics platforms (NGS, qPCR, variant analysis platforms) for assay development, qualification/validation, troubleshooting, and reviewing run QC data.
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PCR, NGS, Mass Spectrometry. Develop and optimize procedures for human specimen sample preparation from tissue, cells, whole blood, plasma and serum for diagnostic assays. PCR, NGS, Mass Spectrometry.
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With minimal oversight from the Supervisor, the Molecular Technologist II will be responsible for the efficient and quality execution of sample accessioning, processing, data generation, and reporting in the clinical laboratory.
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This groundbreaking technology is integral to our product offerings, including one of our flagship products, Unity Complete, the only non-invasive prenatal screen capable of assessing fetal risk for both common recessive conditions and aneuploidies from a single maternal blood sample.
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Experienced in developing and optimizing ddPCR testing, conducting DNA extraction and RNA extraction, running same and library prep for tissue RNA sequencing, and using Illumina platforms for library sequencing.
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The applications team develops solutions that are used across many different labs at Calico: NGS library preparation, sample preparation for mass spectrometry, protein expression and purification, high content screening, and other areas.
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Expertise with a wide range of clinical biomarker sample types (e.g. FFPE tumors, serum, plasma, PBMC, ctDNA) and methods (e.g. IHC, ISH, NGS, Flow cytometry, MSD, Olink, spatial proteomics) is preferred.
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