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Familiarity with commercially available NGS kits for targeted and non-targeted RNA & DNA sequencing. Generate NGS libraries for sequencing of RNA and cell-free DNA. Must be skilled in the art of molecular biology especially in the field of next generation sequencing (NGS), carefully document every aspect of the work, have a strong sense for details, and capable of multitasking in a dynamic work environment.
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Understanding of DNA sequencing techniques (NGS and Sanger) and sequence analysis. Background in molecular biology techniques such as cloning, site-directed mutagenesis, DNA and RNA isolation and purification, sequencing, RT-PCR, and qPCR.
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We have deep expertise in molecular biology, bioinformatics, and Next Generation Sequencing (NGS), and maintain a dynamic, fast-paced work environment with significant opportunities for high-impact contribution and rapid professional growth.
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Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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Minimum of 10 years of experience building and maintaining advanced AI/ML models and experience in computational biology, comparative genomics, NGS workflow and analysis to support R&D. You will apply AI/ML models and other innovative digital solutions to enhance global viral surveillance, strain predictions based on genetic, antigenic, and relevant metadata, mRNA sequence optimization and antigen design, automated synthetic DNA/RNA biology workflows, and other scientific workstreams to support vaccine programs.
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Requirements:Bachelor 's Degree in virology, molecular biology, immunology, or another related field with 2+ years of relevant industrial (preferred) or academic experience or a Master's Degree with some relevant.
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Experience with operation, maintenance, and troubleshooting of liquid handling robots, PCR, general molecular biology tools and NGS library prep devices. As you navigate the daily challenges of orchestrating seamless operations and nurturing the growth of your team, you will play a pivotal role in the DNA revolution.
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Experience with current molecular biology techniques including in vitro transcription and translation, DNA/RNA extraction, PCR, and NGS. Previous research experience with genome editing technologies, nucleases, RNA research, and/or DNA modifying or binding proteins is a plus.
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This role will be part of a team that will be involved in all aspects of the NGS workflow including library preparation, targeted enrichment, and DNA sequencing. ESSENTIAL FUNCTIONSParticipate in all aspects of NGS workflow including sample preparation, library generation, DNA sequencing and data generation.
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Optimize and execute molecular-biological reactions such as PCR, DNA extractions from human brain tissue, and next-generation sequencing (NGS) library preparation. Optimize and execute molecular-biological reactions such as PCR, DNA extractions from human brain tissue, and next-generation sequencing (NGS) library preparation.
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Working knowledge of Next-Generation Sequencing (NGS), tissue culture, and molecular biology is preferred. Performing high throughput molecular biology techniques including PCR, cloning, DNA isolation and Next Gen sequencing.
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Execute and analyze molecular assays designed to determine gene editing outcomes (nucleic acid extraction, PCR, Sanger-sequencing, NGS library preparation, Amplicon-seq, Flow cytometry). Extensive hands-on experience with Molecular Biology (as PCR, qRT-PCR, ddPCR, DNA/RNA and protein preparation), Cloning, mammalian cell culture and transfection is required.
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Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations. Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS.
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DNA extraction and processing DNA for applications including PCR, chromosomal microarray (CMA) and next generation sequencing (NGS) Begin and ensure proper execution of analytic pipelines for both CMA and NGS clinical tests.
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The candidate will have demonstrated technical knowledge in genomics, RNA/DNA biology, bioinformatics, biochemistry, encapsulation, microbial experimentation, and/or molecular biology to complete project requirements and will develop innovative solutions to overcome technical challenges.
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