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Demonstrate track record of productivity with running and analyzing multi-color (8+ colors) flow cytometry, immune cell phenotyping, T cell and NKT cell stimulation assays, cytotoxic assays, ELISA, and cell culture aseptic technique.
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Performs testing in support of product manufacturing, employing manual and automated analytical equipment for a variety of molecular, protein and cell based assays, such as PCR, ELISA, and flow cytometry.
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Experience in assay development, mammalian cell culture, ELISA, Western blotting, qPCR, and experience in standard molecular biology techniques such as cloning, protein expression and purification is preferred.
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BS in biology, biochemistry, molecular biology, biomedical engineering, immunology , stem cell biology or related field and 3+ years experience; Or MS in molecular biology or related field and 2+ years experience.
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Discovery Biology, biochemical/biophysical assays, cellular assays, transient transfection, viral transduction, CRISPR, Cas9, GraphPad Prism, Genedata Screener, R, FRET, ELISA, MSD, flow cytometry.
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Experience in molecular biology techniques: ELISAs, Western blot, protein extraction from cell cultures and animal tissues, and Luminex-based multiplex platform (as a plus) Experience in assessing biomarkers (e.g. Luminex, Western blot, PCR, ELISA ) in animal tissues and cell extracts is preferred.
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Proficient in MSD, ELISA, and PCR is a plus. Education: minimal request: bachelor’s degree in a scientific discipline (Immunology, molecular biology, biochemistry, or related field). As a Scientist in Biomarker Analysis, you will be a key contributor to our bioanalytical team.
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A minimum of 3 years of related work experience developing and performing cell or molecular assay techniques (e.g., qPCR, dPCR, cytokine analysis, ELISA, PBMC isolation, flow cytometry). Doctoral degree in Molecular biology, Biochemistry, Immunology, Molecular Genetics, or related field.
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In vitro biochemical (e.g. ELISA) and molecular (e.g. QPCR) assays is required. Have experience conducting in vitro and ex vivo research in skin biology, using established methodologies and developing new methodologies.
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As part of a great team, our Antibody Development Sr. Scientist will have responsibility for the development of research products centered around antibody-based workflow solutions including Western, ELISA, IF, and single cell biology applications.
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Work closely with Scientists to evaluate antibodies, proteins, small molecules, and drugs by in vitro cell-based assays including cytotoxicity assays, flow cytometry, functional assays, and molecular biology assays, including ELISA, WB, ect.
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Highly desired are skills in systems biology studies via R and Python, human immune cell isolation, ELISA, and other cellular and molecular biology methods applied to human immune cells.
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Perform intracellular cytokine staining/multi-color flow cytometry, antibody neutralization, ELISPOTS, ELISA and other immunological assays. Previous research experience in biochemistry, molecular biology, and tissue culture techniques.
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Strong technical skills or expertise in several of the following areas (ranked by importance): cell culture and transfection, RNA/DNA isolation, quantitative PCR, ChIP, immunohistochemistry and imaging, ELISA/Luminex/MSD, western blotting.
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Demonstrated experience in cell and molecular biology techniques (e.g., cell-based assays, flow cytometry, qPCR, ELISA, and aseptic technique). Position SummaryThe Scientist, QC Analytical (Flow Cytometry) at the Cell Therapy Facility (CTF) is responsible for performing as a subject matter expert in the completion of QC bioanalytical testing or data review for method validation, critical reagent qualification, in-process, release, and stability testing of clinical and commercial cell therapy drug product.
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