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Experience with molecular biology and functional genomics techniques such as CRISPR and siRNA. Experience with the execution of cellular assays at mid and high throughput scale, using a wide range of experimental methods and detection technologies including advanced microscopy, immunoassays (IF, ELISA, Biochemical), functional assays (Electrophysiology, MEA, Calcium Imaging.
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PhD (or equivalent) in bioengineering, biophysics, biochemistry, molecular biology, cell biology, or a related field. Expertise in screening and ranking of antibodies (ELISA, multi-parametric flow cytometry, BLI/OCTET.
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Perform testing of raw materials, intermediates and final products by following analytical methods: Cell based bioassays, Flow Cytometry, ELISA and qPCR assays. Undergraduate degree in microbiology, chemistry, biology or another related science field.
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Experience or knowledge with routine molecular biology techniques (DNA/RNA/protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence) The contractor Research Associate will work closely with the Analytical Development team in the Tech Dev department to help methods development and test samples utilizing PCR, ELISA, Gel Electrophoresis, cell-based and other assays; as well as to contribute to design and performing experiments with supervision as needed; complete assigned tasks within time frames with high quality; maintain a well-documented record of activities in ELN and reports.
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Screen and characterize binders using affinity and activity assays, such as ELISA, FACS, BLI, reporter gene assays. Ph. D. preferred in biochemistry, molecular biology or cell biology with 4+ years post-grad experience, or M.S./B.S. with 6+ years of relevant industry experience.
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Proven experience with assays, such as PCR, ELISA, and mammalian cell-based assays. , ELISA, Gel Electrophoresis, and cell-based assays following SOP and regulatory guidelines. Help to develop and optimize assays for protein, DNA, adeno associated viral (AAV) nanoparticles and related cell-based assay.
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Experience in assay development and validation (e.g. PCR, antibody/antigen ELISA and Western blot) Advanced knowledge in microbiology, virology and/or molecular biology. Experience in assay development and validation (e.g. PCR, antibody/antigen ELISA and Western blot.
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Expertise in molecular biology techniques including RNA extraction, qPCR, ELISA/MSD, and ICC. Candidates with a background in muscle biology will be given preference. Expertise in molecular biology techniques including RNA extraction, qPCR, ELISA/MSD, and ICC.
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Key Words: biology, pharma, qualification, Quality control, quality assurance, technical documentation,workflow, files, document management, auditing, engineering, pharma, pharmaceutical, contract to hire, contract, permanent, full time, benefits, 401K, direct hire, cell culture, gene therapy, temperature mapping, 21 CFR Part 11, remediation, quality systems, process, tanks, filling lines, manufacturing, bioreactor, aseptic, sterile, fill, finish, upstream, downstream.
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Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing. Demonstrated experience of 3-5 years in executing a variety of cell biology techniques, including mammalian cell culture, in vitro cellular assays (e.g., cell viability, proliferation, invasion, apoptosis), and luciferase assay.
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Experience in immunological methods such as ELISA, antibody characterization, and western blot; proficiency in PCR amplification, cloning, protein expression and purification, and tissue culture techniques.
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Independently perform cellular and molecular biology assays including: flow cytometry, ELISA, PCR, and immunocytochemistry. Bachelor of Science degree in Biology, Molecular Biology, Physiology or a related discipline.
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Cell culture assays, flow cytometry and immunocytochemistry, such as DNA/RNA/Protein isolation and electrophoresis, PCR, rtPCR, qPCR, Western Blot, Molecular Cloning, ELISA. Cell culture and differentiation duties may include isolate neural stem cells by mouse brain microdissection, passage, maintain, expand and freeze human and mouse neural and pluripotent stem cells.
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PREFERRED : bachelor's degree in biological sciences; exposure to a research lab; working knowledge of molecular biology and genetics; and experience in at least one of the following: bacterial/mammalian cell culture, PCR, Sanger sequencing, Western blotting, flow cytometry, ELISA, RT-qPCR, histology, and ethical and humane use of animals (especially mice) in research.
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Conduct various in vitro cell biology techniques to include performing flow cytometry analysis of cell surface protein expression, cell adhesion and migration assays, transfecting cells with expression constructs, preparing lysates of cells in culture or tissues, quantification, ELISA assays, SDS PAGE, Western and Immunoblotting and gel electrophoresis.
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