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Tissue Culture work: maintenance of in vitro and ex vivo (primary) cell culture. Operation of antibody-based immunogenicity assays (neutralizing antibody assay and total binding antibody assay.
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Prior experience in bioreactor operation and cell culture media preparation is a must. Must be comfortable with physical activities of constant standing, walking, and sitting with arm stretching into biosafety cabinets for cell culture work and occasionally lifting 50Ibs.
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Previous mouse handling, tissue histology, microscopy, and/or cell culture experience. Tissue sectioning, antibody labeling, and characterization of antibody staining by confocal microscopy.
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Experience with writing and following an institutional animal care and use committee (IACUC) protocols Experience with protein expression and purification, virus-like-particles production and concentration, and RNA vaccines Previous experience with immunization of mice and rabbits Advance skills in molecular biology methods, including cloning and PCR, tissue culture work with different cell lines, and transfection.
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Perform cell culture techniques related to cell-based assays such as antibody-dependent cellular cytotoxicity (ADCC,) microneutralization, Median Tissue Culture Infectious Dose (TCID50) assays would be preferred.
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Experience with some of the following techniques: PCR, RT-qPCR, mammalian cell culture, flow cytometry, transient transfection, affinity chromatography (FPLC), size-exclusion chromatography (HPLC), ELISA, affinity measurements.
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Manufacturing cGMP production activities include solution preparation, master/working cell bank production, upstream cell culture, downstream protein purification, mRNA and molecular therapeutics production, and small-scale fill/finish.
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Specific Duties & Responsibilities Uses molecular biology methods (cloning, PCR assays, mutagenesis), cell culture methods (transfections, virus infections, cell line maintenance), biochemical procedures (protein electrophoresis, western blotting, immunoprecipitation, protein purification, protein micro-array, immunofluorescence), recombinant herpes virus and baculovirus generation.
$15 - $20.4 an hour depends on experienceFull-timeExpandApply NowActive JobUpdated 3 days ago - UpvoteDownvoteShare Job
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Direct experience with upstream development (USP) for biologics such as stable cell line development, cell culture process development (bioreactor) and scale-up. Experience in biologics is required and experience in advanced biologics such as antibody-drug conjugates or cell/gene therapy preferred.
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GMP production activities include, but are not limited to, solution preparation, upstream cell culture, downstream protein purification, mRNA production and filling of Drug Substance. The business includes our proprietary GPEx cell line engineering platform, our new state-of-the-art biomanufacturing facility in Madison, WI, and our market leading biologics analytical services as well as SMARTag Antibody Drug Conjugate technology in Emeryville, CA. Leveraging our growing differentiated technology portfolio, world class manufacturing capability, and other integrated services across the Catalent network, Catalent Biologics is positioned to drive significant growth for Catalent.
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Experience in cell culture and cell-based assay development, T-cell biology, and flow cytometry is helpful but not required. Perform hands-on execution of mammalian cell culture experiments in BSL2 environment including DNA plasmid transfection and small-scale virus production, purification and transduction of cell lines.
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Industry experience in sterile fermentation techniques preferably mammalian cell culture. Take routine analytical measurements for cell growth, gas-content analysis, pH, osmolality, nutrient and metabolite measurements and HPLC analysis of monoclonal antibody titers.
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This includes extensive lab work including mammalian cell culture, flow cytometry, antibody discovery, antibody purification, antibody profiling, and other immunological and protein engineering protocols.
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Experience with cell-based assays, immunofluorescent microscopy, qPCR, flow cytometry, single-cell genomics, RNAseq, cell culture, organoid culture, Illumina NextSeq 550, DNA methylation sequencing, ddPCR, Luminex, clinical chemistry instrumentation, Kryptor, Access 2, robotic liquid handling (e.g. Tecan), Vortex, and DepArray.
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