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Experience with tissue culture, PCR, novel biofluidics assays of blood coagulation, ELISPOT assays, RNA Seq and transcriptomics analysis preferred. Required Knowledge, Skills and Abilities Knowledge of complex laboratory procedures: flow cytometry, western blots, molecular biology, Immunology, and protein chemistry required.
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Innovate workflows for rapid DNA/RNA extraction, next generation sequencing (NGS) and bioinformatic analytics to include engineering detection. Stay abreast of the latest advances synthetic biology, Next Generation Sequencing (NGS) sample preparation methods, sequencing platforms, and bioinformatics.
$50,900 - $89,100 a yearFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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We provide world-class development and manufacture of mammalian and microbial-based therapeutic proteins, plasmid DNA (pDNA), messenger RNA (mRNA), viral vectors, and genetically engineered cells.
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Measurement of molecules in vivo: western blot analysis of proteins, northern blot analysis of RNA transcripts, RT-PCR or Real Time Reverse Transcriptase PCR analysis of RNA transcripts.
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In this vital role you will develop, implement, and execute a global intellectual property strategy for protecting Amgens therapeutic products and product candidates (e.g., antibodies, dual targeting molecules, immunotherapeutics, RNA therapies, or cell-based therapies), including formulations, process development, and manufacturing methods relating to Amgens pipeline and in-line products.
$248,191 - $293,890 a yearExpandUpdated Today - UpvoteDownvoteShare Job
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Ad-Hoc Work products and documents related to preparing blood by whole lysis or density sedimentation methods; prepare cell samples for flow cytometry; extract DNA and RNA from human and murine blood and marrow; perform molecular and cell biologic protocols; maintain bacterial and tissue cultures.
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Knowledge of complex laboratory procedures: flow cytometry, western blots, molecular biology, Immunology, and protein chemistry required. Conducts cell culture and immunological assays, e.g. flow cytometry, ELISPOTs, ELISAs, and serological assays using human and mouse model samples.
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Perform a wide range of molecular biology techniques including qPCR, DNA/RNA analysis, DNA/RNA isolation and purification, next generation (Illumina) sequencing, transfection, and gene cloning.
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Perform, with technical independence, basic and advanced laboratory procedures such as Plasmodium falciparum tissue culture, genetic manipulation, nucleic acid extraction, PCR, qPCR, CRISPR-targeting design, and next-generation RNA and DNA sequence analysis.
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Support lab efforts by routinely employing molecular biology techniques including, but not limited to PCR, qPCR, RNA/DNA isolation, gel electrophoresis, and primer design. Perform high quality cDNA and library construction, including RNA/DNA purification and quality control using various instruments, including Qubit, Bioanalyzer, TapeStation, and Femto Pulse.
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Candidates are expected to be familiar with the following techniques: cell culture, transfections, cloning, DNA and RNA isolation, immunoblotting, PCR, real-time PCR, MTT assays, cytofluorimetry, CRISPR gene editing.
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Prepare DNA, RNA and/or protein from samples, including libraries and PCR. Prepare DNA, RNA and/or protein from samples, including libraries and PCR. Prepare samples for flow cytometry; analyze samples in the flow cytometry core.
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Experience with large scale sequencing analysis (single cell, spatial sequencing, RNA, whole genome sequencing, and/or whole exome sequencing), bioinformatics, and machine learning. Sequencing data to analyze will include spatial sequencing, single cell sequencing, whole genome, bulk RNA sequencing.
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We use multidisciplinary approaches including single-molecule RNA and translation imaging in fixed and live cells, bioengineering methods as well as 3D and in vivo models. Date Posted Friday, January 12, 2024 Position is with Lab/Branch/Program Name of Hiring Official/Investigator Job Description A postdoctoral position is available in the lab of Dr. Stavroula Mili in the Laboratory of Cellular and Molecular Biology, National Cancer Institute, NIH, Bethesda, MD.Dr. Mili's research program focuses on the roles of localized mRNAs in mammalian physiology and tumor progression.
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The contractor shall: Perform bioinformatic support using standard computer software packages for data management including data checking and cleaning of genotype and phenotype data, merging data, creating and recoding variables to prepare data for analyses, analyzing omics (DNA and RNA) data using a variety of standard statistical techniques including classical regression and statistical genetics tools.
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rna job in Mclean, VA
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