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Have knowledge of the execution and implementation of the bioanalytics associated with RNA or AAV gene therapy with gene editing preferred. Develop, qualify, and validate (as appropriate) bioanalytical assays (expressions assays including mRNA and protein [ELISA/IHC], anti-AAV capsid nAb, CTL IFN-γ ELISPOT, IgG/IgM, biodistribution and shedding) for all programs and define science-driven, phase appropriate, and risk-based analytical development strategies to support the development candidate during discovery through preclinical evaluation (including GLP) and clinical development advancement.
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Standard molecular biology techniques: PCR, RT-PCR, cloning, transfection, transduction, DNA/RNA isolation, immunoblotting, and gel electrophoresis. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.
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Work closely with RNA production, LNP/RNP formulation, and in vitro and in vivo team members. Characterize the RNA/RNP encapsulated LNPs for their size, zeta potential, morphology, encapsulation efficiency, integrity, and stability using relevant assays (DLS, TEM, Ribogreen, CE, AGE, PAGE, UV-VIS, HPLC, etc.
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Ability to perform molecular biology techniques, including cloning, DNA extraction, RNA extraction and qPCR is preferred. Hands-on experience with sugar beet transformation is preferred. Ability to perform molecular biology techniques, including cloning, DNA extraction, RNA extraction and qPCR is preferred.
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GreenLight Biosciences is a public benefit company using RNA to create a world where plants, people, and the planet can thrive together. The successful candidate will support the VP of Sales and Marketing in multiple areas of order fulfilment, order to cash, after sales and service, distributor/key account plans and operational management.
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Basic experience with biochemical assays, in vitro assays and molecular biology techniques including mutagenicity, cytotoxicity (determined by any of several methods), genotoxicity, and possibly other endpoints, such as the flow-based micronucleus assay, and RNA isolation.
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Examples are, but not limited to, molecular/DNA/ RNA testing, molecular infectious diseases testing, and next generation sequencing. A bachelor's degree from an accredited college/university, including 16 semester hours of biological science (with one course in microbiology), 16 semester hours of chemistry (with one course in organic or biochemistry) and one course in mathematics, AND two years of post-certification clinical laboratory experience (e.g., blood banking, chemistry, hematology, microbiology, immunology, clinical microscopy, etc.
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Come play an important role in the development and commercialization of an exciting RNA structural dynamics platform and help solve the world’s most challenging diseases. We are a team of serial entrepreneurs and the pioneers of the RNA dynamics field.
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Develop and troubleshoot established and new techniques related to DNA, RNA and protein detection and quantification in rodent and primate brain and placenta (e.g., iDISCO, GeoMX, RNAscope). The current Staff Scientist positions will support research exploring the mechanisms by which endocrine disrupting compounds (EDCs), including phytoestrogens, flame retardants, and BPA (Bisphenol A), alter neuroendocrine pathways in the brain related to sex specific physiology and behavior using a variety of traditional and transgenic in vivo models, in vitro approaches, and a suite of neuroanatomical, neurobehavioral, and molecular testing strategies.
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The Oligo Product Specialist for Cytiva is responsible for providing expert technical leadership during sales process by demonstrating superior product and application knowledge for Cytiva large scale DNA/RNA synthesis products.
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Perform molecular biology techniques such as; PCR, gel electrophoresis, CRISPR guide synthesis, DNA/RNA extraction, etc. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.
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This includes evaluating toxicological mechanisms and responses for target organ systems using multiple strategies related to chemical screening and prioritization including assessing in vivo predictive value of in vitro tests and test methods development and interpretation.
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Willingness and ability to learn and explain clearly developing scientific advances in RNA. Maintain an awareness of the scientific developments surrounding RNAi. Presentations of RNA technology to regulators.
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OR, A bachelor's degree from an accredited college/university, including 16 semester hours of biological science (with one course in microbiology), 16 semester hours of chemistry (with one course in organic or biochemistry) and one course in mathematics, AND two years of post-certification clinical laboratory experience (e.g., blood banking, chemistry, hematology, microbiology, immunology, clinical microscopy, etc.
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Recognized as RNA technical expert within Global Regulatory team. Initiative leadership to address potential concerns in studies, regulator questions etc regarding RNA technology. Address any questions from regulatory authorities on RNA technology.
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rna job in Durham, NC
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