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The ideal candidate will plan and execute production and purification of novel RNA molecules, while demonstrating agility to learn new tools and techniques to characterize purity of the RNA molecules.
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Responsibilities: Formulate and characterize novel formulations for RNA nanoparticles (e.g. small, ultra-small formulation batches, DLS, chromatography)Support flow cytometry evaluation of RNA nanoparticlesIn vitro evaluation of nucleic acid formulation (e.g. mammalian cell culture, transfection, cell assay, etc.
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Skilled in molecular biology techniques (cloning, transfection, transduction, qPCR, CRISPR, RNAi, RNA seq, and western blot). Perform multi-color flow cytometry experiments to analyze immune and/or cancer cells.
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The incumbent will be responsible for the oligonucleotide synthesis, purification, desalting, and analysis to support the process development of guide RNA production in various scales. To accelerate and expand its efforts, CRISPR Therapeutics has established strategic partnerships with leading companies including Bayer and Vertex Pharmaceuticals.
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Processing mouse blood and tissue samples for DNA, RNA, and protein extraction. We are actively seeking an exceptional in vivo pharmacologist to join our team and make significant contributions to our pioneering work.
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The key functions for this lab-based role is to perform cell-based experiments to assess the performance of our novel RNA molecules and to conduct the end-point assays for various in vivo experiments (e.g. ELISA, MSD, flow cytometry.
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Performs assays utilizing standard techniques (antibody staining, flow cytometry, DNA/RNA extraction and analysis, standard and quantitative PCR, ELISA, cloning) Performs assays utilizing standard techniques (antibody staining, flow cytometry, DNA/RNA extraction and analysis, standard and quantitative PCR, ELISA, cloning.
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As part of this job, you will work closely with post-doctoral fellows and senior computational biologists to analyze cutting-edge datasets – including metabolomics, proteomics, RNA sequencing, and whole genome sequences.
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Experience with one or more of the following techniques is also preferred: protein/RNA biochemistry, viral diagnostics, microscopy, CRISPR applications, quantitative data analysis. Experience with one or more of the following techniques is also preferred: protein/RNA biochemistry, viral diagnostics, microscopy, CRISPR applications, quantitative data analysis.
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At the Transcriptomics Platform, this position will work closely with the Platform manager to assist in running samples through an advanced robotics pipeline that creates sequence-ready NGS libraries for both bulk and single-cell RNA sequencing.
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Assessment of treatment effects from both RNA (e.g., bulk, single cell, TCR/BCR, and spatial) and protein modalities (e.g., CITE/REAP-Seq, mIF, and high dimensional flow cytometry. Assessment of treatment effects from both RNA (e.g., bulk, single cell, TCR/BCR, and spatial) and protein modalities (e.g., CITE/REAP-Seq, mIF, and high dimensional flow cytometry.
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Familiarity with molecular biology techniques and experience with nucleic acid extraction, PCR, IHC/IF, NGS, and/or single cell RNA sequencing is a plus. Familiarity with molecular biology techniques and experience with nucleic acid extraction, PCR, IHC/IF, NGS, and/or single cell RNA sequencing is a plus.
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CRISPR, RNA/DNA analyses (RNseq), immunohistochemistry, immunoprecipitation, immunoblotting, and general lab organization/safety. CRISPR, RNA/DNA analyses (RNseq), immunohistochemistry, immunoprecipitation, immunoblotting, and general lab organization/safety.
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Experiences and deep knowledge in the development of genomics medicine (gene transfer, gene up/downregulations and/or gene editing with RNA and/or DNA) using viral and/or non-viral approaches in the industry.
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In addition to basic methods of cell and molecular biology including signal transduction pathways, microRNA and long noncoding RNA, transgenics and gene knock out the Chen Lab has used a number of rodent animal models, in which stem cells, growth factors and advanced matrix scaffolds are applied.
$47,800 - $71,900 a yearFull-timeExpandApply NowActive JobUpdated Today
rna job in Quincy, MA
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