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The successful candidate will focus on advancing antibody discovery efforts using next-generation sequencing (NGS) technologies. Minimum 3 years of hands-on experience with NGS library generation and operating Illumina sequencing platforms.
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Good familiarity with antibody repertoire analysis- NGS data handling and analysis from either in vivo or in vitro sources. Continually improve single-cell RT-PCR, PCR, molecular cloning, and BCR sequence analysis to best harvest variable antibody regions for expression, NGS and/or repertoire cloning into display platforms, with associated analysis and management of data.
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Develop and optimize NGS protocols for library generation, sequencing using Illumina MiSeq/NextSeq platforms, and library QC and analysis. Minimum 3 years of industry experience of hands-on NGS library generation and operating Illumina sequencing platform is required.
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Work closely with NGS discovery team to best strategize and thoughtfully apply NGS based analysis throughout the discovery process and lead for change in establishment of the best feedback loop of discovery/optimization for therapeutics.
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PCR-based typing experience (rSSO, SSP, SBT, or NGS). Day Shift 7:30 am- 4 pm Requirements for the Medical Laboratory Technologist- Histocompatibility:Bachelors degree in biological or chemical science or medical technology or clinical laboratory scienceCertified Histocompatibility Specialist (CHS) certification required (ACHI)Minimum of three years of progressively responsible experience in a Histocompatibility (HLA) testing environment (at least 1-3 years in a lead/supervisory role is preferred.
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PhD, PharmD, or MD in a related scientific field (e.g., genetics, molecular biology, immunology, rare diseases) required. Knowledge of the genetics and pathophysiology of rare diseases.
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PhD with 2+ years’ industry experience in molecular biology, computational biology, genetics, or another relevant field. Perform advanced NGS data analytics to elucidate key biological targets for next-generation CHO host evolution.
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Experience: Preferably experienced with oligonucleotide-based therapeutics, molecular biology, and genetics. Educational Background: Ph. D. in Pharmacokinetics, Pharmacology, Biophysics, Bioengineering, or a related field.
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Doctoral degree in computational biology, biomedical informatics, biostatistics, statistical genetics, genetic epidemiology, or computer science. The laboratory of Dr. Pradeep Natarajan at the Massachusetts General Hospital Cardiovascular Research Center (CVRC) and Center for Genomic Medicine (CGM) and Broad Institute of Harvard & MIT has a unique post-doctoral fellow position open for a highly qualified applicant interested in investigating the genetics and biology of cardiovascular diseases using human genetics across diverse epidemiological cohorts, hospital-based biobanks, and within clinical trials.
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Work closely with and strongly influence the internal Global Biologics teams such as wet lab discovery, NGS, AI/ML groups, to best strategize for and lead for change in establishment of the best feedback loop of discovery/optimization for therapeutics application of scientific expertise in the review of wet-lab and in silico data with team members.
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DNA characterization and quality control testing using techniques such as gel electrophoresis, RNaseA testing, nanodrop, PCR, and preparation of samples for sequencing analysis (Sanger and NGS.
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Developing novel statistical tools to improve the power to identify disease gene association by integrating epigenetics and xQTLs to GWAS. SKILLS & COMPETENCIES REQUIRED: The ideal candidate should have received (or expect to receive soon) a , biostatistics, genetics, computational biology, bioinformatics, computer science and/or related field.
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Broad experience in the application of biochemical and cell-based techniques, such as mammalian cell culture, transfections, qPCR, NGS, dPCR. In addition, the candidate will help design, validate, and implement high throughput NGS and qPCR/dPCR based assays.
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In addition to strong diagnostic skills in surgical pathology, expertise in molecular diagnostics (including NGS and FISH) is desirable. Board certification in Molecular Pathology (including NGS) is a significant plus.
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In this role, you will use various CRISPR delivery systems to execute high-throughput screens, and extract samples for NGS (DNA), RT-ddPCR (RNA) and protein (flow or Western blots/Jess automated blots) readouts to identify lead guides across our internal and external programs.
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ngs genetics jobs in Medford, MA
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