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Experience with standard immunological assays such as flow cytometry / FACS, magnetic cell sorting, ELISA/Luminex, immunofluorescence staining and confocal microscopy, cell proliferation assays, cytotoxicity assays.
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Leverage platforms including our proprietary Mercy Halo assay, ELISA, flow cytometry and western blot to support early development activities including biomarker characterization and reagent screening / optimization.
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Proven expertise in cell culture techniques and a high level of proficiency in molecular biology techniques, including ELISA and PCR. The successful candidate will demonstrate proficiency in a diverse range of molecular and cellular biology techniques, including ELISA, PCR, and other relevant assays.
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Expertise in various analytical techniques for protein characterization (e.g., SDS-PAGE, ELISA, Western blot, protein quantitation, FTIR, DSC, particle analysis). PhD (preferred) in biological sciences, biochemistry, or bioengineering, with a specialization in protein biochemistry.
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Ability to develop immunoassays in some or all of the following immunoassay platforms: ELISA, MSD-ECL, RIA, DELFIA, HTRF, and AlphaScreen. Ability to develop immunoassays in some or all of the following immunoassay platforms: ELISA, MSD-ECL, RIA, DELFIA, HTRF, and AlphaScreen.
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Demonstrate strong attention to detail, familiarity with animal care policies, and a proactive willingness to acquire new skills to enhance and expand their current skill set, while also having proficiency in biomarker assays such as colorimetric/enzymatic assays, ELISA, Western blot, PCR/qPCR/ddPCR, etc.
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays to determine protein purity and function. The incumbent will work at the bench to express, purify and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner.
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Execute routine procedures based on bioassay methodologies, such as plate based assays (ELISA, qPCR, ddPCR, ribogreen), gel electrophoresis, western blot and cell based assays. This candidate will be responsible for laboratory activities in support of process development and industrialization and ensure the characterization of mRNA. These analyses include HPLC, ELISA, qPCR, western blot and more that support the process development.
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Primary duties include 3-4 steps synthesis of novel lipids, lipid-nanoparticle formulations and characterization, cell culture, blood collection, tissue harvesting, RT-qPCR, ELISA, and flow-cytometry; imaging techniques such as bioluminescence and fluorescence; and general lab support, e.g., ordering and maintaining solvents/chemicals stock.
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Proficiency with standard bioanalytical and molecular biology techniques including ELISA, qPCR, absorbance spectroscopy, pH, and conductivity. Proficiency with standard bioanalytical and molecular biology techniques including ELISA, qPCR, absorbance spectroscopy, pH, and conductivity.
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Cell culture, mammalian cell culture, RNA, circular rna, HPLC, FPLC, Elisa, gel electrophoresis, Capillary gel electrophoresis, IVT, biochemistry, protein purification, PCR, qPCR, liquid handlers, hamilton.
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Biochemistry: Conduct standard biological or biochemical assays and laboratory analyses, e.g., TR-FRET, FP, AlphaScreen, ELISA, and cell-based assays, etc. The Center for Protein Degradation (CPD) is seeking a biologist/biochemist to join the Biochemistry and Structural Biology team.
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Uses standard research equipment which may include, but not be limited to the following instruments: centrifuge, ultracentrifuge, coulter counter, luminometer, label maker, ELISA reader, Gel Doc, spectrophotometer, and PCR machine; 6.
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Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc. Additionally, bench-level experience in biochemical assays such as Western analysis, PCR and RT-PCR, immunohistochemistry, ELISA, luminescence, etc.
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elisa job in Cambridge, MA
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