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Extensive expertise in RNA, protein, and peptide analysis particularly in biopharmaceutically relevant contexts. Optimize and validate analytical and bioanalytical methods for the characterization of RNA molecules, including but not limited to purity, identity, integrity, and stability assessments.
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About AlnylamAlnylam Pharmaceuticals (Nasdaq: ALNY) has led the translation of RNA interference (RNAi) into a whole new class of innovative medicines with the potential to transform the lives of people afflicted with rare and more prevalent diseases.
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Preferred with at least 1 year molecular lab experience including DNA/RNA extraction, library preparation and NGS analysis. Preferred with at least 1 year molecular lab experience including DNA/RNA extraction, library preparation and NGS analysis.
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Conduct RNA, DNA, and epigenomic sequencing experiments. A multi-disciplinary team of researchers led by Dr. Gabriel Griffin at the Dana-Farber Cancer Institute (with co-affiliation with the Broad Institute of MIT and Harvard) seeks a Research Technician to join efforts to find new antibody-based therapies for cancer.
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You will have deep technical savvy, a strategic mindset, and clear, forwarding-thinking leadership in AI/ML, computational biology and molecular biology, with a proven track record of leveraging computational tools to drive innovation in biotechnology, particularly in the realm of molecular biology, genome/transcriptome-wide analysis, single cell analytics, molecular design and structure, next generation sequencing, to predict RNA structure, regulation, and splicing.
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The key function of this lab-based role is to lead multiple steps in the manufacturing of RNA, including DNA production, RNA in vitro transcription, RNA purification and RNA analytics to support key preclinical studies of new RNA therapeutics.
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Direct experience analyzing RNA sequencing and other types of genomic data sets is strongly desired. Sanofi is seeking an innovative investigative toxicologist to join our Preclinical Safety organization in a position that offers exciting and challenging scientific opportunities in a global environment.
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Routine tests may include virus RNA extraction, PCR, DNA sequencing, real-time PCR, plasma and PBMC isolation from whole blood, PBMC subpopulation isolation, and drug susceptibility assays with HIV-1 and HIV-2.
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Essential qualifications:- Experience in basic molecular biology techniques, including: PCR, DNA/RNA extractions, solution preparation- Good hands in lab and dedication to hands-on laboratory work- Ability to lead a project independently and problem solve as needed- Ability to work well with others in a team environment- Ability to multi-task and prioritize in an environment with multiple demands and ongoing projects- Excellent organizational skills and a strong attention to detail.
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Cell culture, mammalian cell culture, RNA, circular rna, HPLC, FPLC, Elisa, gel electrophoresis, Capillary gel electrophoresis, IVT, biochemistry, protein purification, PCR, qPCR, liquid handlers, hamilton.
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Performs techniques such as tissue culture, RNA isolation, PCR, immunohistochemistry, electrophoresis, protein purification and others. Under close supervision of Principal Investigator, conducts a variety of routine experiments following established procedures and protocols, which may include performing assays, tissue culture, RNA isolation, electrophoresis, and other laboratory techniques.
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Ability to develop, evaluate, and implement workflows for technology, optimize and standardize existing techniques and workflows with respect to experimental HT mRNA generation process workflow including DNA & IVT-RNA production, and in vitro/in vivo screening is desired.
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The person will perform animal husbandry including genotyping and weaning pups, animal experiments including T cell transfer and tumor implantation, laboratory experiments including tissue culture, RNA and DNA preparation, qRT-PCR, tissue preparation and histological analysis, flow cytometry on a routine basis.
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As part of this job, you will work closely with post-doctoral fellows and senior computational biologists to analyze cutting-edge datasets including metabolomics, proteomics, RNA sequencing, and whole genome sequences.
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RNA Scientist (Protein Engineer) Operate in-house processes for circular RNA (circRNA) and mRNA production, including running HPLC or FPLC, collecting and pooling chromatogram peaks, and reporting in LIMS.
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rna job in Boston, MA
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