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Doctoral degree in computational biology, biomedical informatics, biostatistics, statistical genetics, genetic epidemiology, or computer science. The laboratory of Dr. Pradeep Natarajan at the Massachusetts General Hospital Cardiovascular Research Center (CVRC) and Center for Genomic Medicine (CGM) and Broad Institute of Harvard & MIT has a unique post-doctoral fellow position open for a highly qualified applicant interested in investigating the genetics and biology of cardiovascular diseases using human genetics across diverse epidemiological cohorts, hospital-based biobanks, and within clinical trials.
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Independently performs non-routine, highly specialized experimental procedures, as well as, carries out a wide range of moderate to complex genomics techniques, including DNA/RNA extraction, next-generation sequencing, NGS library preparation/target enrichment, microarrays, and Sanger sequencing.
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At least 2 years' laboratory experience working in NGS, with a focus on liquid handling and automation being preferable (HTS, Assay Development, or molecular or structural biology backgrounds will also be considered.
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Innovate : Implement new technologies (nanopore, NGS library optimization, etc) or in-vivo antibody discovery. You have knowledge of NGS library generation. Family planning support up to $45,000 (e.g., IVF/PGT, adoption, surrogacy, egg retrieval.
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Understanding of nucleic acids DNA/RNA analytical performance assessment tools: PCR, Cloning/plasmid generation and design, Quality control on DNA/RNA (capillary electrophoresis, UV quantification, Sanger sequencing, NGS.
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Independently design, execute and troubleshoot various biochemical and cell-based assays to further the company's understanding of oligo-directed RNA editing including, but not limited to enzyme kinetics, protein purification, protein binding assays (SPR, fluorescence polarization), structural biology, molecular modeling, SHAPEmap and RNA folding, LC-MS, cell-based assays, RNA isolation, Western blotting, qPCR, dPCR and NGS.
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This group studies the basis of autoimmune disease using techniques in human genetics, bioinformatics, and systems biology. The self-motivated applicant will work in the Raychaudhuri Lab within the Department of Medicine, Division of Genetics.
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Experience with CryoEM and/or X-ray crystallography. Using an oligonucleotide-based approach, Korro expects to bring its medicines to patients by leveraging its proprietary platform with precedented delivery modalities, manufacturing know-how, and established regulatory pathways of approved oligonucleotide drugs.
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Proven experience working with complex matrices and cell isolation, ideally also exposure to technologies required for cell and gene therapy products such as NGS, PCR variations including ddPCR, flow cytometry.
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Candidates should have proven experience in oligonucleotide analytical qPCR, RT-qPCR, and/or NGS method development, quality control processes and hand-on experience with state-of-the-art analytical instruments.
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Designing in-house NGS based genomic experiments for range of applications in target identification and validation, lead characterization, and biomarker discovery and validation. POSITION : Scientist/Senior Scientist/Principal Scientist in Computational Biology.
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Operate and maintain automated liquid handlers for plasmid purification, as well as downstream QC applications like NGS and DNA normalizations. Conducting routine DNA characterization and quality control testing using techniques such as gel electrophoresis, fragment analysis, nanodrop, endotoxin testing, and preparation of samples for sequencing analysis (Sanger and NGS.
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In addition to strong diagnostic skills in surgical pathology, expertise in molecular diagnostics (including NGS and FISH) is desirable. Board certification in Molecular Pathology (including NGS) is a significant plus.
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Experience with RNA/DNA NGS and automation is a plus. Molecular biology laboratory techniques including bacterial culture, PCR, cloning, protein blot analysis, assay development (western blot, ELISA, luminescence), and phage propagation.
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Design and execute experiments using standard molecular biology techniques including PCR, cloning, DNA purification, and preparation of NGS samples from various display libraries. Design and execute experiments using standard molecular biology techniques including PCR, cloning, DNA purification, and preparation of NGS samples from various display libraries.
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ngs genetics jobs in Boston, MA
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