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Postdoctoral fellow will be responsible for recruiting patients from clinic, collecting blood samples, processing blood, harvesting peripheral blood monocytes and performing immunologic assays such as ELISAs, ELISPots, flow cytometry, tetramer assays, rt-PCR, gene expression analysis, cell culture and tissue processing/staining.
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Solid use of wet lab techniques such as in vitro enzyme incubations, cell culture, and micropipetting. The individual will be expected to have good organizational skills, attention to detail, and solid experience in wet lab work; enzyme incubations, in vivo sample handling, and/or organic extractions preferred.
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Hands on proficiency with common molecular biology bench techniques; cell culture experience. Single cell laboratory experience is highly desirable. As a Field Application Scientist (Chicago metro area), you will be engaging with customers daily and delivering system usage trainings at customer sites, while providing experimental design, data analysis, logistical, and troubleshooting support across your region.
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Ensure compliance with all applicable regulatory safety standards in the laboratory departments, including cell culture, molecular, NGS, histology, biorepository, and nucleotide extraction laboratories across 3 sites.
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Design and perform advanced in vitro and in vivo experiments, including the use of mammalian cell culture, CRISPR gene editing, lentiviral transduction, immunoblotting, immunohistochemistry, and animal procedures.
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Performs research with molecular/cell biological and histological techniques including PCR, DNA and RNA extraction, immunostaining, Western-blotting, cell culture, and preparing cryosections.
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Knowledge of clean room protocols, drug discovery, process development, cell culture technologies and GMP environments would be advantageous. Welcoming Culture: Mutual support, team spirit and international collaboration; communities on numerous topics such as "Coaching.
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We offer great opportunities to learn new techniques in next generation sequencing, stem cell biology, and mitochondrial metabolism. Examples of assays include ELISA, Western blot, viral plaque assay, flow cytometry, and PCR.
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Next generation sequencing (NGS) sample preparation, cell culture, flow cytometry. This key position will manage and conduct research and development projects related to 1) genomics investigations of cancer samples and development of novel genomics methodologies for oncology diagnostics and 2) tumor immunology projects focused on anti-neoantigen T cell responses with an aim towards the development of personalized cellular immunotherapies.
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Experience and knowledge with the following techniques: molecular biology techniques including PCR, plasmid construction, site directed mutagenesis, and recombinant protein expression and purification and cell biology techniques including cell culture, DNA transfection, and cell-based assays.
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Experimental work will include cell culture, molecular biology, and maintenance (breeding/genotyping) of mouse colonies. We are committed to not only recruiting, but also retaining a diverse, mission driven workforce and enabling a culture of inclusiveness in an environment that values service excellence, stewardship, personal well-being, and professional development for all of our employees.
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We utilize mouse and cell culture models of viral and bacterial infection, and a variety of immunological, microbiology, molecular biology and biochemical techniques to achieve these goals.
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Tissue Culture techniques (i.e. ex vivo cultures, primary cell isolation, transfection, infection, co-culture, three-dimensional modeling). Cancer stem cell isolation and culture (human or mouse tumors.
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Will perform experiments and performing analysis utilizing advanced microbiology and immunology skills in cloning, genetic manipulations, data analysis, statistical analysis, animal infections, ELISA assays, cell culture and primary cell growth, handling of human blood, cell sorting, fluorescent microscopy, biochemistry, and protein purification.
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Some of the related techniques that may be applied in the research to be conducted will include: microinjection, mass spectrometry, high-throughput screening, mammalian cell culture, cell transfection assays, flow cytometry (FACS), immunoprecipitation/Western blots.
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cell culture jobs in Chicago, IL
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