Associate Scientist II

Job Title: Associate Scientist IILocation: Watertown, MADuration: 12 Months (Possible Extension or Conversion into Permanent) TOP SKILLS NEEDED PER HIRING MANAGER:Mammalian cell culture (including isolation of hematopoietic cells from human bio samples, culture & differentiation of human primary hematopoietic cells, generation of edited lines via eg CRISPR/Cas or overexpression)Tissue sample processing (protein and mRNA) for downstream bioassaysGenerating and analyzing cell-based/molecular assay data using a variety of methodologies (examples: qRT-PCR, plate-based assays, enzyme assays) with rigorous scientific standards, troubleshooting where needed, and careful documentation practicesMammalian cell culture experienceMaster's Degree + min of 2 years working experience preferredPURPOSE:The Rare Disease Research team is looking for a highly motivated Associate Scientist to support early-phase research aimed at developing novel therapies for patients with rare blood disease. The candidate will be responsible for cell and molecular biology work supporting vitro and ex vivo studies to identify and validate new targets.REQUIREMENTS:• MS in biology + 2 years working experience (PREFERRED) or BA in biology + 4 years’ experience.• 2+ years of relevant laboratory experience (industry setting preferred, not academic)• Hands-on experience with mammalian cell culture. (Preferred: experience with isolation and culture of primary human hematopoietic cells, experience generating edited cell lines)• Experience in downstream bioassays including one or more of the following: qPCR, ELISA/Western blot, multicolor flow cytometryThanks,