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Advanced knowledge of cell culture, cell line development, bioreactor operation and scale-up, centrifugation and ultracentrifugation, tangential flow and normal flow filtration. The ideal candidate must have excellent tissue culture skills (human cell line and stem cell culture preferred), be trained in GMP grade culture and formulation of various human cell types including peripheral blood mononuclear cells, T-cells, dendritic cells, multi potent stem cells such as mesenchymal stromal cells, pluripotent stem cells, such as human embryonic stem cells and induced pluripotent stem cells, project and personnel management skills and experience in quality control tests.
$6,566.67 - $14,583.33 a monthFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Description Under the supervision of the Director of the Human Gene and Cell Therapy Facility (HGCTF), the HGCTF Lead Manufacturing Associate is responsible for the manufacturing of human gene and cell therapy products under Good Manufacturing Practices (GMP), quality control testing of these products to ensure that the products meet the required quality, supervise other manufacturing associates and overall management of all projects.
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Experience with protein characterization, electrophoresis, chromatography, aseptic techniques, cell culture, LAL and assay validation/qualification. Perform and review routine wet chemistry/raw material assays and ELISA/cell-based immunoassays, electrophoresis (SDS-PAGE/IEF) and HPLC.
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Experience in cell line development, ELISA, PBMC biomarker, cell culture, cellular assays, gene expression is desirable. Perform cell biology and molecular biology studies to support early drug discovery projects.
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Experience in confocal microscopy, advanced molecular biology, genomic data analysis, and mammalian cell culture are highly desirable. Experiments will involve cutting-edge cell and molecular biology techniques including differentiation of iPSCs and iPSC-derived RPE cells, spatial transcriptomes/ multiomics analysis, andadvanced microscopy imaging tools.
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Preferred qualifications: Familiar with techniques such as multi-color flow cytometry and/or mass cytometry, sterile technique for cell culture with BSL-2+ practices, ELISA, qRT-PCR, and sterile technique for bacterial culture.
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The RI will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
$19.5 - $29.87 an hourExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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The individual in the role will perform flow cytometry, immunoassay, cell culture, molecular assays, analyze and review experimental data, and carefully document work. Basic immunology knowledge, hands on experience in cell culture, knowledge in PCR, ELISA and cell sorting.
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Masters in Chemical Engineering, Biochemical Engineering, Life Sciences or Biotechnology, or Bachelors in Chemical Engineering, Biochemical Engineering, Life Sciences or Biotechnology with 2+ years of relevant cell culture lab experience.
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3-5 years of experience performing cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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Process human blood and tumor samples and/or perform a variety of experiments (e.g., molecular cloning, mammalian cell culture, flow cytometry, ELISA, xenograft mouse models) independently or under the supervision of senior lab members.
$20.18 - $26.44 an hourFull-timeExpandUpdated Today - UpvoteDownvoteShare Job
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Individuals with a scientific background, mammalian cell culture and cGMP experience needed. Conducting routine manufacturing of biologic products including media preparation, cell culture, cell counting, formulation, centrifugation and freezing.
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This will be accomplished using a variety of technologies including pain behaviors, confocal microscopy imaging, live calcium imaging, mast cell and neuronal cell culture, cell and molecular biology and analysis with ELISA. This position will involve method development, data analysis and preparing the data for presentation and publication.
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Tissue and cell culture work, viral and cell line passaging and manipulation. Demonstrated experience in common molecular, biochemical and cellular biology techniques in laboratory environment required (including tissue culture, in vivo experiments.
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Proficiency in molecular biology, protein-interaction, immunochemistry, and cell culture. Lead and perform lab-based experiments that include preparing primary neuronal cultures, live cell imaging, protein-interaction assays, kinase activity assays, biochemical analysis of synaptic proteins, immunocytochemistry, mouse electroencephalography, slice electrophysiology mouse seizure scoring, mouse behavior.
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cell culture jobs in Santa Monica, CA
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