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Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography. Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography.
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We work with a variety of systems to model the interactions between T cells, cancer cells, and other immune cells both in vitro and in vivo. Experience with mammalian tissue culture and flow cytometry.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing.
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This includes the phenotypic analysis of engineered CAR-T cells by flow cytometry, and the functional characterization of T cells via methods such as cytotoxicity, proliferation, and MSD / other cytokine secretion assays in vitro.
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Kindbody's clinically managed program includes fertility assessments and education, fertility preservation, genetic testing, in vitro fertilization (IVF), donor and surrogacy services, and adoption, as well as physical, mental, and emotional support from preconception through postpartum.
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Investigate and validate mechanism of action of drug leads by conducting in vivo, ex vivo and in vitro pharmacology experiments. The position may also involve in ex vivo and in vitro pharmacology experiments.
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Design and run in vitro and in vivo validation assays of TCR targets. Skilled in Assay Development, Cell isolation, Cell engineering, Gene editing (CRISPR, Lentiviral, Retroviral) This post is to recruit another scientist in immuno-oncology to join our interdisciplinary team.
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Experience with complex in-vitro model systems is highly desired. Experience with immortalized and/or primary cell culture, cell viability assessment, Luminex/ELISAs, gene expression analysis, high content screening, confocal microscopy, and flow cytometry is highly desired.
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Designing, coordinating, executing, and analyzing in vitro and in vivo experiments. Developing in vitro functional assays for the characterization of allogeneic cell therapy candidates. Experience in planning and executing in vitro functional assays to characterize primary immune cells including, but not limited to, cytotoxicity assays, cytokine/chemokine measurements, phenotypic characterization, and proliferation assessment.
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Prepare semen for intrauterine insemination (IUI) and in vitro fertilization (IVF) is a leading fertility clinic network and family-building benefits provider for employers offering comprehensive virtual and in-person care.
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Develops, validates and executes biochemical and cell-based assays to characterize and screen biologic therapeutics in vitro and ex vivo, to support lead-finding/optimization efforts. Experience with multi-color flow cytometry, T cell functional assays, ELISA assays, culture of mammalian cell lines and primary immune cells is required.
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Scientific tasks include development and execution of experiments involving PCR, qPCR, in vitro fermentation, headspace gas chromatography, cell culture with rich or freshwater media, protein expression in bacteria, cell harvesting, protein characterization.
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Conduct literature search to design in vitro/in vivo models and conduct thorough research to initiate pipeline development. Mammalian cell culture, cytotoxicity analysis and multi-color fluorescence flow cytometry.
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Responsibilities:We are currently seeking a Director, Drug Metabolism and Pharmacokinetics, who will have scientific oversight of one or more of the small molecule discovery projects and will manage the in vitro and in vivo PK, ADME and bioanalytical assays.
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Experience with common immunological lab techniques and methods, such as: functional in vitro and ex vivo immune cell assays, immunoprofiling by flow cytometry, cytokine secretion assays. Experience with common immunological lab techniques and methods, such as: functional in vitro and ex vivo immune cell assays, immunoprofiling by flow cytometry, cytokine secretion assays.
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vitro job in Alameda, CA
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