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Experience with multi-color flow cytometry, panel design, primary cell culture, cell sorting and/or data analysis is preferred. Develop in vitro cell-based assays (cell culture, gene editing, flow cytometry, high content imaging, cytokine profiling, cytotoxicity, proliferation, etc.
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The selected candidate will perform laboratory and investigative procedures by conducting a variety of experimental techniques such as molecular cloning, cell culture (cell lines and primary cells), flow cytometry, CRISPR gene editing, and analysis of mouse tumor models.
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Access to a network of expert Flow Cytometry and bioanalytical scientists to collaborate with and grow. Unlimited opportunities to solve Flow Cytometry and Biomarker challenges and help bring novel therapies to market.
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Proficiency with standard cellular and molecular biology techniques including gel electrophoresis, western blot, ELISA, flow cytometry, chromatography (size exclusion, ion-exchange), and qPCR is required.
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Participate in the construction and screening of immune-based libraries through immunization, antibody display, and flow cytometry. Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Standard immunological techniques - Flow cytometry, ELISAs / Elispot, fluorescent microscopy Molecular biology skills - PCR, rt-PCR and RNA work, basic cloning Three published first authored papers in immunology.
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Serve as Subject Method Expert to facilitate the development, validation, transfer, and trending of Cell therapy flow cytometry and potency analytical methods. Shape strategies for cell therapy product characterization using various flow cytometry and potency analytical platforms.
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Knowledge of general lab assays is required (e.g., Cell culture, ELISA, Flow Cytometry, Molecular Biology), but experience is preferred. In addition, this individual will conduct some routine characterization testing, using various laboratory methods (e.g., ELISA, Molecular Biology.
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These tasks may include molecular biology assays such as PCR and cloning; mammalian cell culture experiments; flow cytometry assays; isolating RNA and performing qPCR; and mouse genotyping and assays in mice.
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Performs in-life and post-life immunological assays including high parameter (16-35+ colors) immunophenotyping/intracellular cytokine staining flow cytometry and analysis, FACS, Luminex cytokine profiling and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
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Experience with multi-color flow cytometry and high throughput assays such as CTG, HiBiT, or live cell imaging techniques such as Incucyte. Experience with standard cell and molecular biology experimental design and techniques such as cell culture, lentiviral production, stable cell line generation, western blot, and qPCR.
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Executes experiments and analyzes data from flow cytometry analytical assays with limited supervision, as part of the Cell Therapy Analytical Development (CTAD) team. Flow cytometry assay development experience is preferred.
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Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types. Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot.
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The successful candidate will contribute to the day-to-day operations of the flow cytometry core and maintaining a high-functioning laboratory capable of meeting the needs of client’s Oncology, Inflammation, and Virology therapeutic discovery groups.
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Working knowledge of flow cytometry, molecular biology, and genetic engineering is a plus. Experience in flow cytometry, microbiology, PCR and ELISA testing is a plus.
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molecular postdoc flow cytometry jobs Title: post doctoral fellow Company: Emory University
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