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Serve as Subject Method Expert to facilitate the development, validation, transfer, and trending of Cell therapy flow cytometry and potency analytical methods. Shape strategies for cell therapy product characterization using various flow cytometry and potency analytical platforms.
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Executes experiments and analyzes data from flow cytometry analytical assays with limited supervision, as part of the Cell Therapy Analytical Development (CTAD) team. Flow cytometry assay development experience is preferred.
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The selected candidate will perform laboratory and investigative procedures by conducting a variety of experimental techniques such as molecular cloning, cell culture (cell lines and primary cells), flow cytometry, CRISPR gene editing, and analysis of mouse tumor models.
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Experience with multi-color flow cytometry, panel design, primary cell culture, cell sorting and/or data analysis is preferred. Develop in vitro cell-based assays (cell culture, gene editing, flow cytometry, high content imaging, cytokine profiling, cytotoxicity, proliferation, etc.
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Standard immunological techniques - Flow cytometry, ELISAs / Elispot, fluorescent microscopy Molecular biology skills - PCR, rt-PCR and RNA work, basic cloning Three published first authored papers in immunology.
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The successful candidate will already have experience with basic laboratory skills and will receive training in a broad array of techniques such as the preparation of NGS libraries, single cell sequencing via droplet capture on the 10X Genomics platform, and flow cytometry.
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Access to a network of expert Flow Cytometry and bioanalytical scientists to collaborate with and grow. Unlimited opportunities to solve Flow Cytometry and Biomarker challenges and help bring novel therapies to market.
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An important part of the job will relate to the development of multi-color (10+) Flow Cytometry assays. Prior experience in flow cytometry is a must experience in immunology and tissue culture is a plus.
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Leverage platforms including our proprietary Mercy Halo assay, ELISA, flow cytometry and western blot to support early development activities including biomarker characterization and reagent screening / optimization.
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Performs in-life and post-life immunological assays including high parameter (16-35+ colors) immunophenotyping/intracellular cytokine staining flow cytometry and analysis, FACS, Luminex cytokine profiling and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
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Experience carrying out retro-orbital bleeds and intravenous and intraperitoneal injections, isolation and preparation of mouse tissues for antibody staining, and analysis by flow cytometry.
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Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques. Experience working with flow cytometry and standard molecular biology techniques.
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The qualified candidate will utilize systems immunology techniques including flow cytometry, cell culture, bacterial culture, single cell RNA sequencing, cytokine analysis, cell isolation, and data analysis.
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Piper Health Sciences is seeking a Research Associate II to join a pre-clinical organization focusing on in vivo models of human diseases located in Rockville, MD. The Research Associate II will perform flow cytometry and other immunological assays.
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Conduct a real-time flow cytometry assay in support of increased manufacturing process control and consistency in viral growth kinetics. Experience with flow cytometry and/or performing immunoassays is preferred.
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disease vaccine flow cytometry jobs Title: post doctoral fellow Company: Emory University
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