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Performing a variety of basic immune and cancer cell biology assays, including in vitro proliferation or apoptosis assays, drug sensitivity assays, flow cytometry and western blotting, in vivo xenograft experiments.
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Responsibilities will include assisting in the in vitro characterization of the pharmacology of the radiopharmaceuticals by performing techniques like tissue culture, protein expression and purification, immunoblot, flow cytometry, biolayer interferometry, qPCR. Moreover, this role will require direct animal handling, which will include establishing and maintaining an animal colony of mouse models of human cancer, and small animal imaging and treatment studies.
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Responsibilities include cell culture and characterization, standard molecular biology techniques (PCR, RNA extractions, amplification, and purification), and coordinating interactions with other labs.
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They will perform research activities in the fields of cell and molecular biology such as in vitro cell culture work (including culture of human breast cancer cells), isolation of primary cells from human tissues, molecular cloning, protein quantification, western-blot, DNA/RNA extraction, and Q-PCR, amongst other.
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Responsibilities may include Experimental design; performing and analysis of complex in vivo and in vitro experiments; animal experiments and husbandry; immunology, cell biology, and multi-omic experimental knowledge and analysis (i.e. flow cytometry, single cell RNA sequencing, multiplex immunohistochemistry analysis); manuscript preparation and grant writing.
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Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Principal Scientist, Oligonucleotide Biology- RNA, Gene Therapy & Delivery located in La Jolla, CA.
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Good written/oral communication skills in English, and experience in cellular and molecular biology techniques (e.g., flow cytometry, RNA isolation, protein analysis methods), rodent models and cell culture in vitro models are highly desirable.
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Measurement of molecules in vitro: Electrophoretic mobility shift assay (EMSA) for protein-DNA or protein-RNA interactions, Co-immunoprecipitation to detect protein-protein or protein-RNA interactions.
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Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays. Additionally, will perform molecular biology work including cloning, primer design, Gibson Assembly, restriction enzyme digestion, ligation, site directed mutagenesis, in vitro transcription, and reverse transcription.
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ResponsibilitiesLead a team of experts and technicians to develop a differentiated LNP-based delivery system for RNACollaborate with the RNA team to conduct in vitro and in vivo studies, leading to eventual clinical trialsImprove upon the current mainstream LNP-based delivery systems, to increase the expression rate, lower the immunogenicity, etc.
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Expertise is required with how RNA secondary structure and topology affects both its generation (in vitro or in vivo) and its function. Your primary role involves management and hands-on research including purification process development and analytical development / characterization of mRNA and circRNA, with the ultimate aim of producing therapeutic-grade material for in-vitro downstream assays and deliver scalable purification strategies to reduce immunogenicity and maximize in-vivo expression.
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Molecular experience in PCR, ddPCR, DNA/RNA extractions/purification is a plus. A basic understanding of Virology and In vitro virus culture experience is a plus. ATCC, a pioneer in biomaterials manufacturing, is seeking a Biologist to work onsite in the BioProduction Virology department in Manassas, VA. Prior experience with mamamalian cell culture and aseptic technique is required.
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Support flow cytometry evaluation of RNA nanoparticles. Formulate and characterize novel formulations for RNA nanoparticles (e.g. small, ultra-small formulation batches, DLS, chromatography.
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PhD in immunology and 1-2 years of experience in translational research including RNA biology, in vitro models of human lung disease, and bioassays. PhD in immunology and 1-2 years of experience in translational research including RNA biology, in vitro models of human lung disease, and bioassays.
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Perform in vitro functional studies of ncRNAs biomarker candidates in neuronal and glial cells differentiated from human induced pluripotent stem cell (hIPSC) Basic molecular assay (RNA isolation, qPCR.
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