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Proven expertise in cell culture techniques and a high level of proficiency in molecular biology techniques, including ELISA and PCR. The successful candidate will demonstrate proficiency in a diverse range of molecular and cellular biology techniques, including ELISA, PCR, and other relevant assays.
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The individual will be an integral team member and contribute to the understanding of molecular and cellular phenotypes of in vitro and in vivo models of neuromuscular diseases, discovery and characterization of potential therapeutic compounds using relevant models.
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Experiences in cell and molecular biology and immunological techniques, including cell culture, Western immunoblotting, QRT-PCR, gene transfection and cloning, RNA interference and DNA sequencing, flow cytometry-based assessment of immune system mechanisms that regulate cancer initiation, promotion and progression, the role of the tumor microenvironment in cancer immunity and novel approaches to cancer immunotherapy.
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Techniques may include any or all of the following: Mammalian cell culture, Sterile technique, In vitro cell based assays (e.g. reporter gene bioassay, cell titer glo proliferation bioassay, etc.
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Experience with standard molecular biology techniques such as PCR, bacterial plasmid production, in vitro transcription. Perform experiments to produce DNA templates for in-vitro transcription.
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Sample quality control (QC), next-generation sequencing (NGS) library prep and target enrichment, microarrays, CRISPR, PCR/qPCR, bioreagents, and data analysis solutions including interpretation, and reporting.
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We are a recently established translational leukemia research laboratory that uses a variety of biochemical, molecular, and cell biology approaches to identify new therapeutic targets, develop preclinical models of disease, and test novel therapeutics both in vitro and in vivo using mouse models.
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We are seeking a highly motivated and talented scientist with background in cellular and molecular biology and assay development to join the Biotherapeutics and Genetic Medicine organization to support in vitro pharmacology work related to Genetic medicine research on AAV.
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Experience culturing bacteria, and especially anaerobic bacteria; with protein expression, especially in vitro protein expression; with molecular biology, particularly digital PCR; and with automated liquid handling.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry. Cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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Molecular experience in PCR, ddPCR, DNA/RNA extractions/purification is a plus. A basic understanding of Virology and In vitro virus culture experience is a plus. Health & Wellness: Comprehensive medical coverage and company paid Life Insurance, Disability Insurance & AD&D Work life balance with Paid Holidays and PTO Fitness and cell phone subsidies, and additional benefits such as Aflac, legal services, and pet insurance Employee Assistance Program offering around-the-clock counseling.
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The specialist will perform in vitro cell culture assays, qRT-PCR, molecular biology cloning, CRISPR gene editing, biochemistry, and next generation sequencing assays including single cell genomics.
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Must have experience with diagnostic, clinical and immunological including but not limited to ELISA, multiplex immune assays such as Luminex, Quanterix or MSD, qRT-PCR, cell culture, piccolo tests, etc.
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In addition, Laboratory Technicians have the technical training and experience required to perform regulated clinical laboratory test realization (test specimen receipt through test result generation) using standard in-vitro laboratory techniques in Molecular Biology (PCR) and in Mass Spectrometry.
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This person will closely interact with the principal investigator and work on research focused investigating the mechanistic roles of protein machinery involved in protein degradation (e.g. proteasome) using various in vitro and in vivo methods (e.g. mechanistic enzymology, cell culture & C. elegans.
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Building a candidate pipeline through a great internship program for local college students and recent graduates at local universities is a great and cost-effective way to attract and retain top talent. By offering meaningful and impactful work experiences, regular feedback, coaching, and mentorship, you can create a positive internship experience that will make your organization a sought-after destination for future employees. This not only benefits the organization in the short-term but also in the long-term, as you'll have a pool of well-trained and experienced candidates who may be interested in full-time employment once they graduate. Furthermore, building relationships with local universities and college students can increase brand awareness and build a positive reputation for your organization in the local community.