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Mammalian cell culture, cytotoxicity analysis and multi-color fluorescence flow cytometry. Conduct literature search to design in vitro/in vivo models and conduct thorough research to initiate pipeline development.
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Must possess some but not all of the following laboratory skills: tissue culture, PCR, Western blots, flow cytometry, and immunostaining / immunofluorescence microscopy and analysis, cryostat sectioning.
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Essential laboratory skills including molecular cloning, bioproduction, RNA and protein analysis, tissue culture, fluorescence and confocal microscopy, flow cytometry/sorting and animal handling are highly desired.
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Expertise in the following techniques is mandatory: magnetic cell isolation, cell culture, phenotyping of bone marrow progenitors using multicolor flow cytometry, CFU assays using CD34+ cells, ELISA, MSD, Western blot for signaling pathway proteins, basic molecular biology including isolation of nucleic acid and real-time PCR.
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Flow cytometry, fluorescent microscopy, suspension cell culture, lentivirus generation, SDS-PAGE and western blot, viral titering by TCID50, cell-based assays, ELISA, affinity chromatography, complement assays, Prism software.
ExpandApply NowActive JobUpdated 9 days ago - UpvoteDownvoteShare Job
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Experience in flow cytometry, ELISA with preferred experience in MSD and/or Luminex. Working with multiparameter flow cytometry, including cell surface staining, intracellular staining, data acquisition and analysis.
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The ideal candidate has direct experience with mammalian tissue culture, protein purification, proficiency in standard chromatography techniques, protein characterization (e.g., MS, SEC), and binding assays (e.g., ELISA and flow cytometry.
ExpandApply NowActive JobUpdated 11 days ago - UpvoteDownvoteShare Job
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Proficient in basic cell characterization techniques, including flow cytometry, ddPCR, qPCR, ELISA. Experience in CRO and/or scientific team management is an asset. Key Responsibilities: Lead preclinical studies that provide scientific rationale for advancement to human trials, including safety, efficacy, and mechanisms of action studies using clinically relevant in vitro and in vivo models.
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Two or more years experience in conducting immunological assays including immunophenotyping/flow cytometry on a diverse assortment of primary immune cells, FACS, Luminex cytokine profiling and analysis, magnetic bead enrichments for population analysis, ELISpot, and ELISAs.
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Direct experience performing in vitro functional assays is preferred, including cytotoxicity, proliferation, multi-color flow cytometry and cytokine release. Perform phenotypic analysis of engineered CAR T cells using multicolor flow cytometry.
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Develop and implement functional assays, including IncuCyte, eSight, RTCA, ELISA, MSD, and multicolor flow cytometry. Lead the design, development, and optimization of T-cell candidates using in vitro and in vivo assays.
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Hands-on experience in Cell Culture, ELISA/MSD, Flow Cytometry, PCR (NGS is a plus) Under supervision, execute and then lead development activities for cell-based methods, flow cytometry, qPCR / ddPCR and ELISA.
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Relevant experience with T cells and other immune cells in vitro and in vivo, immunological techniques, and multicolor flow cytometry are highly desirable. CRISPR, RNA/DNA analyses (RNseq), immunohistochemistry, immunoprecipitation, immunoblotting, and general lab organization/safety.
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Molecular biology techniques: DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, Next Generation Sequencing, flow cytometry. Cell biology techniques: Mammalian cell culture, in vitro cellular assays (cell viability assay, proliferation, invasion, apoptosis), luciferase assay.
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Our laboratory is recruiting a postdoctoral fellow to use unbiased approaches (RNSeq, scRNSeq, spatial profiling, and flow cytometry) to identify novel molecular and cellular events in solid cancers with different treatments.
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