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Experience with wet lab approaches, including biological sample processing, ELISA, qPCR, in vitro cellular and molecular approaches, including cell culture, protein analysis with BCA and Western blot, immunostaining, microscope imaging, and flow cytometry.
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This position additionally requires providing technical support for in vitro and in vivo experiments, encompassing molecular biology, cell culture, and flow cytometry, for the benefit of post-doctoral fellows and graduate students within the laboratory.
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The candidate's main responsibilities will include: a) novel assay development, b) in-vitro T cell circuit design and validation, c) establishment of in-vivo tumor models, d) compiling pre-clinical results to support the designation of clinical vector design, and e) leading the in-vitro and in-vivo pharmacology characterization of therapeutic product candidates in collaboration with a process development team towards an IND filling.
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Our faculty currently have strengths in gene expression, signal transduction, epigenetics, chromatin and structural biology, novel technology development, pharmacology, systems modeling, and in vivo models to study mitochondrial dynamics, cellular stress response pathways, mechanisms of genomic alteration, novel treatments, disease development, Epithelial-Mesenchymal Transition, phenotypic plasticity, and chemoresistance in cancer.
$76,333 - $109,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Candidates with experience performing bacterial culture for protein expression and cloning, protein purification using chromatography systems, protein characterization using SDS - PAGE , UV-Vis., and fluorescence spectroscopy, as well as running in vitro and cellular assays to evaluate the activity of proteins will be preferred in the selection process.
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In South San Francisco is seeking an Associate Principal Scientist with strong expertise in complex cellular model/assay development, mechanistic receptor pharmacology, and lead discovery to join our Quantitative Biosciences (Pharmacology) department.
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We develop the next generation of imaging technology, molecular imaging, in-vitro diagnostics, image-guided therapeutics, and informatics for precision health. The program of work at Stanford focuses on developing new IBD animal and cellular models, and testing novel compounds and cell therapies in preclinical studies.
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Good written/oral communication skills in English, and experience in cellular and molecular biology techniques (e.g., flow cytometry, RNA isolation, protein analysis methods), rodent models and cell culture in vitro models are highly desirable.
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The experiments involve both in vivo, ex-vivo, whole animal, and in vitro approaches. The Penn State College of Medicine, Department of Cellular & Molecular Physiology and the He laboratory, in Hershey, PA conducts NIH funded research projects with focus on the mechanisms involved in vascular inflammation, atherosclerosis, vascular disease-initiated Alzheimer's development, and the development of prevention and therapeutic strategies.
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We strive to dissect the molecular, cellular, and tissue-level mechanisms by which genetic variants modulate disease risk, with a major focus on the role of microglial cell function in vitro and in vivo.
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Mosaic teams provide comprehensive, end-to-end discovery services, including program assessment and strategy development; program management; antibody discovery and optimization; protein production, purification, and characterization; protein engineering; biochemical and cellular assay development; formulation, stability and developability studies; bioanalytical (PK and PD) assay development; and candidate-validating pharmacology.
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Demonstrated expertise and hands on experience with in vitro antibody display systems (yeast, phage, mammalian, and/or nucleic acid display). PhD in Protein Engineering, Molecular Biology, Biochemistry, Genetics or related discipline, with 10+ years of industry experience.
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Contribute to the project development as well as perform cellular immunological techniques, including multi-color flow cytometry, ELISA, MSD and in vitro immune cell assays. The Amgen Translational Immunology and Safety Sciences group based in South San Francisco, CA, is seeking a Principal Scientist to independently investigate immune related safety assessment with in vitro approaches and provide immuno-toxicological evaluation strategy to support Inflammation pipeline projects.
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They will also participate in the translational efforts aimed at characterizing therapeutic targets by investigating the mechanisms of action of new molecules using in vitro and ex vivo cellular models.
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The candidate should have a track record confirming proficiency in basic immunological and molecular biological techniques, such as FACS (multicolor staining, PBMC isolation, expansion and in vitro differentiation; BD software; FlowJo, GraphPad), MACS, ELISA, ELISPot, aLISA, WB, qPCR. Experience in in vitro assays of monoclonal antibodies or small molecules as well as knowledge of bioinformatics and metadata analysis methods a plus.
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