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Experience with HPLC/UPLC-based assays or molecular-based assays is a must (i.e., qPCR/ddPCR, SDS-PAGE, fluorometric assays), desire to expand analytical expertise highly preferred. The Quality Control Manager/ Sr. Manager will be responsible for QC laboratory operations and GMP method performance of analytical chemistry and molecular based assays.
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3-5 years of analytical experience in protein/peptide separations by CE-SDS and/or cIEF in pharmaceutical or biotechnology company. Process Development, Stability and Characterization analytical support, optimization, and qualification in a range of analytical techniques including U/HPLC, capillary and plate based techniques.
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Extensive hands-on experience in a wide range of analytical techniques, such as UV-Vis, HPLC, CE, SDS-PAGE, icIEF, LCMS, GCMS, enzyme kinetics, colorimetric and fluorescent plate-based assays, ELISA, Western Blot, etc.
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The Analytical Development Scientist position is a technical position responsible for supporting Analytical and Process Development utilizing early and late phase capillary methods including electrophoresis and Isoelectric focusing in support of DS and DP process development.
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Extensive expertise in U/HPLC, CE, SDS-PAGE, mass spectrometry, and other analytical technologies as applied to protein analytical method development, trouble shooting and validation for GMP release and stability testing.
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Protein purification (AKTA, HPLC, SDS-PAGE, BLI/SPR) Bachelor’s in Molecular Biology, Synthetic Biology, Biochemistry, Structural Biology, Protein Chemistry, or similar field. Molecular biology (primer design, Gibson, Golden Gate, Ligation, CRISPR.
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Testing may include ELISA, BCA, Spectrophotometry, HPLC, SDS-PAGE, ddPCR / PCR, Nova BioFlex, and Vicell Counting assays. Testing may include ELISA, BCA, Spectrophotometry, HPLC, SDS-PAGE, ddPCR / PCR, Nova BioFlex, and Vicell Counting assays.
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Antibody characterization/QC by SDS-PAGE, HPLC-SEC, and Gator to determine antibody quantity and quality. Support antibody expression construct generation by molecular cloning, DNA sequencing, and plasmid DNA purification.
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Hands-on experience with at least two techniques, such as HPLC, capillary electrophoresis, ELISA, or SDS-PAGE, used for analysis of recombinant proteins. Looking for 2-4 yrs of cGMP experience, further details and interest apply to the below Responsibilities The QC Analyst 2 will contribute to the success of the operation by providing accurate, GxP-compliant analyses of clinical biopharmaceutical products and by participating in assay qualification and validation studies.
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays to determine protein purity and function. The incumbent will work at the bench to express, purify and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner.
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Knowledgeable in several of the following areas: HPLC, UPLC, iCE, CGE, MS, ELISA, SDS-PAGE, Biopharmaceutical Testing, Karl Fischer, UV-Vis, FT-IR, TOC. Laboratory experience involving CE, HPLC, ELISA and compendia.
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Demonstrated experience with analytics and characterization of proteins and antibodies (SDS-PAGE, Western Blot, residual Host Cell Proteins, ELISA residual endotoxin, residual DNA, HPLC.
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Experience with QC techniques and technology of Cell and Gene Therapy products including: flow cytometry, cell-based assays (i.e., Potency);dPCR/qPCR; HPLC; CE-SDS or SDS-PAGE. Experience with QC techniques and technology of Cell and Gene Therapy products including: flow cytometry, cell-based assays (i.e., Potency);dPCR/qPCR; HPLC; CE-SDS or SDS-PAGE.
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Creation of Empower reports including custom calculations for HPLC, CE-SDS and icIEF technology. HPLC, CE or icIEF experience in a cGMP. Creation of Empower reports including custom calculations for HPLC, CE-SDS and icIEF technology.
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Biochemistry (e.g., ELISA, HPLC, western Blot and SDS-PAGE); plant biotechnology (e.g., chlorophyll measurements, photosynthetic condition assays, plant cell culture) The selected candidate will conduct research and development for 1) assessing the effects of physical, chemical and/or biological agents (singly or in combination) on aquatic organisms (emphasis on reef invertebrates and algae); 2) engage in research to elucidate molecular and physiological bottlenecks to coral reproduction in support of coral reef restoration and delisting of ESA species; and 3) conduct research involving coral disease, diagnosis and treatment.
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