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Execute routine analytical tests and quality control assays, such as qPCR, ELISA, SDS-PAGE, Western blot, and HPLC, to assess the purity, potency, and safety of AAV vector products. Experience with molecular biology, biochemistry, or cell biology techniques and analytical methods (e.g., qPCR, Elisa, Western Blot.
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Ligland binding technology platforms (ELISA & MSD & Luminex)- Aseptic Cell Culture Techniques- Knowledge in basic Protein chaacterization (SDS Page Gel & Western Blots)- Expertise using SoftMac PRO and GraphPad Prism Software.
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Expertise in various analytical techniques for protein characterization (e.g., SDS-PAGE, ELISA, Western blot, protein quantitation, FTIR, DSC, particle analysis). PhD (preferred) in biological sciences, biochemistry, or bioengineering, with a specialization in protein biochemistry.
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Hands-on experience with at least two techniques, such as HPLC, capillary electrophoresis, ELISA, or SDS-PAGE, used for analysis of recombinant proteins. QC Analyst 2 will contribute to the success of the operation by providing accurate, GxP-compliant analyses of clinical biopharmaceutical products and by participating in assay qualification and validation studies.
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Skilled in the latest analytical technologies and methodologies commonly used in the analysis and characterization of biologics, carbohydrates, conjugates and small molecule drug candidates and successful track record of method development trouble shooting and validation, e.g. UV-Vis, various modes of HPLC, CE, SDS-PAGE, icIEF, LCMS, GCMS, SEC-MALS, enzyme kinetics, colorimetric and fluorescent plate-based assays, ELISA, Western Blot, etc.
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Preform testing using HPLC, GC, spectroscopy, LC/MS, ICP, CE/SDS, icIEF, SDS-PAGE, ELISA, diverse immunoassays, and wet chemistry techniques. Proficient with HPLC, GC, LC/MS, ICP, CE/SDS, ELISA, icIEF techniques.
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Experience with ELISA, qPCR, ddPCR, SDS-PAGE, and other non-cell based methods. Experience in basic molecular biology (i.e. RNA,/DNA/plasmid extraction, qPCR, ddPCR, etc.) Transfer of test protocols from Analytical development lab (or clients) to cGMP compliant QC laboratory.
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Perform analytical assays on various vaccine process intermediates, products and related experimental samples utilizing one or more of the following techniques: Separation-based assays (HPLC, UPLC, CE, SDS-PAGE)Plate based characterization (ELISA, enzymatic activity) Absorbance (UV-vis) and fluorescence protein quantificationLaboratory responsibilities include preparation of buffers and testing reagents.
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A strong background in protein chemistry will be essential to perform and troubleshoot techniques such as HPLC, SDS-PAGE, cell-based bioassays, capillary electrophoresis, SDS-PAGE, ELISA, and standard compendial assays.
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays to determine protein purity and function. The incumbent will work at the bench to express, purify and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner.
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Experience in bioanalytical techniques including SEC, CEX, CE-SDS, icIEF, ELISA, and subvisible particle analysis for parenteral drug products is preferred. AbbVie Biologics Analytical Research & Development (Biologics ARD) is a global analytical sciences organization responsible for all CMC analytical activities related to the development of AbbVie's therapeutic biologic products.
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Main instruments used are HPLC, SDS-Page, ELISA, and Electrophoresis, and cell-based bioassays (need experience with at least 2 of the techniques). Type of Experience: SDS-Page, ELISA, and Electrophoresis, and cell-based bioassays (need to have experience with at least 2 techniques.
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Biochemistry and protein chemistry experience (flow cytometry, FACS, ELISA, western blotting, SDS-PAGE, etc.) Designs and executes cell-based and biochemical assays to validate engineered cell lines and targets through flow cytometry, reporter assays, ELISA, western blotting, and cytotoxicity assays to select lead preclinical candidates.
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Perform a variety of wet chemistry and biological routine tests for in process, finished product (SDS PAGE, western blot, ELISA based assays) stability samples and any other additional samples as required.
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Conduct in vitro biological assays including cell culture (viability, proliferation, immunohistochemistry), protein analysis (SDS-PAGE, Western Blot and ELISA) and DNA/RNA protocols (DNA gels, qPCR etc.
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