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Weutilize state-of-the-art technical modalities including flow cytometry, ELISA, protein and DNbased molecular biology, biochemistry, cell/tissue culture, fluorescence and confocal microscopy, and DNA/RNA sequencing.
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We are looking for a dedicated and motivated Research Associate to join a cross-disciplinary team to help develop and support RNA delivery formulation and new RNA product research and development.
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Direct experience with messenger RNA is preferred. Support developing assays for formulation characterization in vitro. Bachelor's degree in molecular biology, chemistry, biochemistry, pharmaceutical science, chemical engineering or related field, Master is preferred.
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Functional characterization (in vitro DNA cleavage) of differential CRISPR-Cas RNP complexes by electrophoretic mobility shift assay (EMSA) or anion exchange (AEX) chromatography. The candidate will join the Process Science analytical team to study RNA folding computationally and experimentally for its CRISPR by Design platform.
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Experience in working with in vitro viral transduction assays, FACS and various cell/molecular techniques (transfection, RNA/protein analysis, immunostaining) are preferred. Performing in vitro cell-handling experiments, including cell transfection, and cell transduction with viral vectors.
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Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing. Demonstrated experience of 3-5 years in executing a variety of cell biology techniques, including mammalian cell culture, in vitro cellular assays (e.g., cell viability, proliferation, invasion, apoptosis), and luciferase assay.
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Work collaboratively and transversally to assist project team members in understanding toxicologic and pharmacologic relevance and implication of in vivo and in vitro study findings as necessary and assist in addressing queries from regulatory agencies.
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Ph. D. in a scientific discipline (e.g., molecular biology, cell biology, or related field) with 0-2 plus years of hands-on experience with gene editing, site-specific nucleases (especially RNguided nucleases), transposons, or other vectors used in vivo or in vitro, is required.
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Applying a range of molecular biology techniques, including molecular cloning, DNA and RNA isolation, and protein expression analysis. We work with a variety of systems to model the interactions between T cells, cancer cells, and other immune cells both in vitro and in vivo.
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By combining in vivo and in vitro models of tissue growth (epithelial, connective, and nervous tissues) and regeneration together with state-of-the-art methods from cell biology, mechanobiology, bioengineering and more, the Nicoli lab has begun to unpick the mechanisms that underpin tissue formation and resilience against changing environments.
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In-silico RNA structural modeling and alternative RNA structure ensemble deconvolution. We are seeking a Scientist with experience in RNA biochemistry and an adaptable skillset of computational, biophysical, and biochemical techniques to probe the structure/function of single-guide RNAs (sgRNAs.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing.
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Experience in various cell and molecular biology techniques including, cell culture (primary and stable cell lines), transfection, stable cell line generation, MSD, ELISA, RNA purification, RT-qPCR, western blots, and other standard cell-based or biochemical assays.
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Develop in vitro & in vivo screening assays in collaboration with therapeutic area scientists by generating cell lines, developing lentiviral & RNP delivery methods, & integrating & optimizing screening protocols.
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Details can be immunology/cell biology related various biological assays, analyzing patients’ samples and tumor-specific effector cells function, molecular biology related to cloning, DNA/RNA extract, and transduction to lentivirus or retrovirus vectors, and assisting with other lab tasks as needed.
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