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Approaches that we use to address these issues include molecular biology, virology, cell and tissue culture, drug susceptibility assays, flow cytometry, deep sequencing, and integrated single-cell analyses.
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MERFISH provides transformative insight into a wide range of tissue-scale basic research and translational medicine in oncology, immunology, neuroscience, infectious disease, developmental biology, and regenerative medicine.
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3 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting. 4 years of experience with molecular biology techniques and in vitro assays, including DNA/RNA extraction, qPCR, immunofluorescence, and Western blotting.
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Weutilize state-of-the-art technical modalities including flow cytometry, ELISA, protein and DNbased molecular biology, biochemistry, cell/tissue culture, fluorescence and confocal microscopy, and DNA/RNA sequencing.
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In addition, the ideal candidate has experience in functional genomics, including the design of pooled genome-wide and custom-library screens with amplicon-seq and single-cell RNA sequencing readouts.
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Specialist appointed at the Full rank must have a PhD (or equivalent degree) in biology, pharmacology, chemistry, bioengineering, or a related field or at least 10 or more years of experience with Molecular biology including RNA and protein-based assays animal models and in vivo work including tissue harvest, specimen collection, histology, and blood processing.
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Hands-on experience with downstream screening methods including high-throughput plate assays, DNA/RNA extraction and sequencing, and more. Experience executing in vivo experiments and/or handling tissue samples.
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Expertise in molecular & cellular biology, tissue engineering, or regenerative medicine and most preferably in skin biology. Experience with standard biochemical and molecular biology techniques, including RNA isolation, qPCR, microscopy, ELISAs, and/or quantitative protein analysis.
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Work products and documents related to preparing blood by whole lysis or density sedimentation methods; prepare cell samples for flow cytometry; extract DNA and RNA from human and murine blood and marrow; perform molecular and cell biologic protocols; maintain bacterial and tissue cultures.
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Basic knowledge of mouse neuroanatomy, genetics, flow cytometry, RNA sequencing, electrophysiology and/or cell biology. Processing mouse, human, and non-human primate brain tissue. Research Associate I - Tissue Processing.
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By combining in vivo and in vitro models of tissue growth (epithelial, connective, and nervous tissues) and regeneration together with state-of-the-art methods from cell biology, mechanobiology, bioengineering and more, the Nicoli lab has begun to unpick the mechanisms that underpin tissue formation and resilience against changing environments.
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Required Qualifications: BS/BA and/or MS/MA in a related field and 2+ years of related experience Experience in tissue culture (especially induced pluripotent stem cells) Experience in molecular biology techniques (e.g. RNA/DNA purification, PCR, cloning, etc.
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Knowledge and experimental ability to perform procedures used in a molecular biology research laboratory setting such as sterile technique, DNA and RNA preparation, protein purification, PCR amplification, bacterial transformation, western blotting, cloning, bacterial growth and handling, plasmid preparation.
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Details can be immunology/cell biology related various biological assays, analyzing patients’ samples and tumor-specific effector cells function, molecular biology related to cloning, DNA/RNA extract, and transduction to lentivirus or retrovirus vectors, and assisting with other lab tasks as needed.
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Performs basic and advanced molecular and cellular biology techniques including: PCR, tissue culture, DNA/RNA/Protein extraction, and processing tissue for immunotyping. Performs basic and advanced molecular and cellular biology techniques including: PCR, tissue culture, DNA/RNA/Protein extraction, and processing tissue for immunotyping.
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