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Support antibody expression construct generation by molecular cloning, DNA sequencing, and plasmid DNA purification. Antibody characterization/QC by SDS-PAGE, HPLC-SEC, and Gator to determine antibody quantity and quality.
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Research Associate, Protein Science, protein expression, purification, microbial cell culturing, molecular cloning, biochemical synthesis, FPLC, SDS-PAGE, ELISA, colorimetric assays, Microsoft Office.
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays to determine protein purity and function. The incumbent will work at the bench to express, purify and QC proteins using biochemical methods such as ELISA, SDS-PAGE, Western, and analytical methods such as FPLC and HPLC. The candidate will be responsible for protein production, data analysis and presenting data to their supervisor and to the team in an effective and timely manner.
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The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Experience with protein characterization and protein purification such as SDS-PAGE, IMAC and/or ion exchange purification. This committed internship position will be responsible for developing small to medium scale high throughput VHH nanobody protein expression in E. coli cells and VHH protein purification.
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General knowledge on protein purification, protein conjugation, HPLC analysis, SDS-PAGE analysis and mass spectrometric analysis of protein. The purification group is responsible for non-GMP protein- drug lead generation, supporting the various research units and their pre-clinical therapeutic in- vitro and in- vivo needs.
$39.44 - $45.77 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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The ideal candidate will have excellent practical laboratory experience in developing scalable polysaccharide purification processes utilizing column chromatography, tangential flow filtration, and related chemistry techniques.
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The successful candidate will have hands-on experience with cell-based in-vitro potency assays, SDS-PAGE, Automated Western blot assays, enzyme activity measurements, protein extraction, purification, and quantification methods.
$72,000 - $90,000Full-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Characterize recombinant proteins using SDS-PAGE, WB, protein quantitation, HPLC, ELISA and other biochemical/biophysical assays such as BLI and SPR to determine protein purity and function. Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is preferred.
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A strong understanding of cell culture methods - Potency, Agarose gel electrophoresis, SDS-PAGE, and cell banking is necessary. Support method development, transfer and qualification/validation activities associated with the cell-based potency assays for AAV-based gene therapy products.
$72,000 - $90,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Skills: HPLC, SOP, In Vitro, In Vivo, Protein Purification, GMP (Good Manufacturing Practice) The purification group is responsible for non-GMP protein- drug lead generation, supporting the various company research units and their pre-clinical therapeutic in- vitro and in- vivo needs.
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Characterize recombinant antibodies/proteins using SDS-PAGE, protein quantitation, HPLC, ELISA and other biochemical assays to determine protein purity and function. Bachelor's or Master's degree in Biochemistry, Chemistry, or a related field with a minimum of 3 years of protein purification experience.
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Strong scientific understanding in analytical methods to characterize large molecules purification such as SDS-PAGE, HPLC, WES, CE etc. Senior Specialist, Engineering, Protein Purification page is loaded.
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Experience with protein purification using an AKTA system and protein characterization using HPLC (analytical SEC), SDS-PAGE, and mass spectrometry. Perform standard protein QC, including SDS-PAGE, analytical SEC, and intact mass spectrometry.
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Experience with recombinant protein expression and purification using mammalian cell lines is preferred. This is a unique opportunity wherein the candidate will be responsible to assist multiple novel research projects along with in vitro and in vivo mAb discovery campaigns.
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