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Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable. Execute large-scale protein purifications using Immobilized metal affinity, ion-exchange, and size exclusion chromatography.
Full-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Bachelor's or Master's degree in Biochemistry, Chemistry, or a related field with a minimum of 3 years of protein purification experience. Experience with recombinant antibody and protein expression and purification using mammalian cell lines is desirable.
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The specific role(s) of the specialist will include: 1) validating CRISPR-ki cell lines and primary cells; 2) affinity purification, chemical cross-linking, and sample preparation for mass spectrometry (MS) analysis; and 3) enzymatic and virological-based assays for functional characterization of protein components.
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Perform expression and purification of recombinant viral protein and human monoclonal antibodies and fragments ( E.coli, insect cells, mammalian cells) via affinity chromatography and FPLC-based size exclusion and ion exchange.
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Process development and manufacturing experience includes mammalian and/or microbial cell culture and scale up, the isolation and purification of proteins, antibodies, etc., extensive experience with product recovery (depth filtration, centrifugation, MF), TFF (UF/DF), chromatography (Affinity, IonX, HPT, HIC.
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Experience with different protein purification approaches such as affinity (His-tag, Fc-fusion), cation- and anion-exchange, size-exclusion chromatography (SEC). Knowledge of AKTA instrumentation and a broad range of purification techniques (affinity chromatography, ion exchange, size exclusion, mixed mode.
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Prior experience in molecular biology and protein expression/purification in an academic or industrial setting. Follow established protocols and carry out small- and large-scale protein purifications (affinity, ion exchange, and size exclusion chromatography.
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Preferred technical expertise in one or more of the following areas: GPCR signal transduction, proximity labeling, affinity purification, in vivo viral transduction, mouse models of metabolic disorders, CRISPR, and multi-omics research.
$76,773 - $87,171 a yearFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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The successful candidate will have hands-on experience with biophysics, chemical biology and/or protein biochemistry techniques (e.g., SPR, MST, DSF, affinity purification, alphaLISA, mass-spectrometry.
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Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is preferred. The ideal candidate will have hands-on experience working with mammalian cell culture, protein expression, purification and analytical QC using diverse biochemical methods such as ELISA, SDS-PAGE, Western blotting.
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Approaches include gain- and loss-of-function genetics coupled with phenotypic analysis, phosphoproteomics, enzymatic analysis of kinase and phosphatase activities, and identification of protein interaction networks using yeast 2-hybrid and affinity purification-mass spectrometry approaches.
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Reporting to the Director of internal Downstream development and manufacturing, focusing on protein purification, disposable technologies and compliance for early-stage clinical production. Hands-on support for downstream purification steps such as chromatography, harvest clarification and normal flow filtration.
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Experience with protein purification (Affinity, AEX, SEC, etc.) An ideal candidate will have AAV purification experience, or broad recombinant protein and vector production experience, a collaborative spirit and a desire to work in a fast-paced startup environment.
$82,000 - $124,000 a yearFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Execute small- and large-scale protein purifications using immobilized metal affinity, ion-exchange, and size exclusion chromatography. Experience with recombinant protein expression and purification using mammalian cell lines is preferred.
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Ability to use HPLC and conventional approaches, such as ion exchange and affinity chromatography, for protein purification of recombinant protein or protein complexes as well as native protein complexes.
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purification affinity jobs
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