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Perform some ion exchange chromatography, buffer and reagent preparation, SDS Gel Electrophoresis, bio-conjugations, and use HPLCs (AKTAs) Perform Protein A/G purification of monoclonal and some polyclonal antibodies.
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In addition, the candidate must be skilled in protein/peptide sample preparation techniques including BCA/NanoDrop protein quantification, SDS-PAGE, and in-gel and solution proteolytic digestion protocols.
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At least 2 years postgraduate, hands-on experience in mammalian cell culture and protein biochemistry such as SDS-PAGE, ELISA and affinity chromatography are required. The Research Associate will be responsible for conducting hands-on experiments including but not limited to molecular biology, mammalian cell culture, recombinant protein purification and characterization.
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Reports to: Staff Scientist, Protein Process Development. Our client is looking for a highly-skilled, motivated, and creative analytical scientist to join their Vaccine Product Development organization as Scientist I within Protein Development and Clinical Manufacturing.
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Hands on proficiency and with protein analysis using HPLC, CE-SDS, Western blotting, Spectroscopy, etc. Vaxcyte is looking for a highly skilled, motivated, and talented analytical scientist to join Vaxcyte’s Vaccine Product Development organization as Senior Associate Scientist within Protein Development and Clinical Manufacturing.
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While working with the Purification Manufacturing team, the Scientist I will be an integral member of the team responsible for purification of monoclonal antibodies from hybridoma cultures using primarily affinity chromatography, as well as the production of fluorescently labeled bioconjugates.
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Experience in protein analysis such as SEC, HPLC, SDS-page, CE-SDS, and cIEF and binding kinetics is a plus. The Company is re-engineering the way highly complex immunizations are made through modern synthetic techniques, including advanced chemistry and our exclusively licensed XpressCFTM cell-free protein synthesis platform.
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Perform experiments within the team: Execution and understanding of protocols for protein chemistry and target validation, such as SDS-PAGE, ELISA, western blots. Apply molecular and cell-based assays including but not limited to RNA transfection, RNA & protein isolation, gene expression analysis (RT-qPCR & RNA Sequencing), western blot, and fluorescence immunocytochemistry.
$110,000 - $140,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Protein Purification and Characterization: execute protein purification techniques including HPLC and CE-SDS. - Minimum of three years of experience in protein purification and characterization.
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A solid grasp of protein chemistry is necessary to use techniques like HPLC, SDS-PAGE, cell-based bioassays, capillary electrophoresis, ELISA, and standard compendial assays. Possess a strong foundation in protein chemistry and experience in resolving analytical issues.
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1 year in molecular biology techniques with hands on expertise in DNA/RNA extraction, PCR, q-PCR, mutagenesis & assembly reactions, SDS-gel page, electrophoresis, cloning replication - pDNA techniques, western, northern, southern blotting, different cell line cultures, affinity chromatography, ELISA, microfluid liquid handling system, and thin layer chromatography.
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Expertise in various analytical techniques for protein characterization (e.g., SDS-PAGE, ELISA, Western blot, protein quantitation, FTIR, DSC, particle analysis). PhD (preferred) in biological sciences, biochemistry, or bioengineering, with a specialization in protein biochemistry.
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Familiar with protein separation, protein purification, and protein detection methods (e.g., HPLC, capillary electrophoresis (CZE, cIEF, CE-SDS) microfluidics) or automation is desirable.
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Deep technical experience in multiple key technical aspects of immunoassay development; including but not limited to protein chemistry, antibody screening, antibody conjugation, assay diluent formulation; multiplexing; preanalytical sample processing; scale-up, assay optimization and troubleshooting; Real- Time PCR, immunoassays (immunoblotting and ELISAs), and gel-based assays (SDS-PAGE and CE-SDS); multivariate data analysis; analytical validation; clinical validation.
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Preferred hands-on experience with molecular cloning and bioanalytical techniques, such as PCR, SDS-PAGE, LC-MS, Western blot, ELISA or other immunoassays, etc. Perform protein expression in bacterial systems, including molecular cloning, fermentation in bioreactors/shake flasks, and protein separation and immunoassays.
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