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Summary: Vaxcyte is looking for a highly skilled, motivated, and talented analytical scientist to join Vaxcyte’s Vaccine Product Development organization as Senior Associate Scientist within Protein Development and Clinical Manufacturing.
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Overview Performs any basic to complex Analytical assay(s) according to and in compliance with Good Laboratory Practices (GLP), current Good Manufacturing Practices (cGMP), Code of Federal Regulations (CFR) and/or Points to Consider (PTC) guidelines and Standard Operating Procedures (SOPs.
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Strong background in protein chemistry and experience troubleshooting analytical results. A strong background in protein chemistry will be essential to perform and troubleshoot techniques such as HPLC, SDS-PAGE, cell-based bioassays, capillary electrophoresis, SDS-PAGE, ELISA, and standard compendial assays.
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Background in organic-polymer synthesis and characterization, biocompatible polymers, peptides and lipid-protein-conjugates, oligonucleotides, structure-property relationships, formulation and process development, small & large molecules, analytical separations, isolation and identification of impurities.
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Lead analytical development and quality control for AAV gene therapy, small molecules, antisense oligonucleotides, mRNA and protein therapeutics. Modalities include AAV gene therapy, small molecules, antisense oligonucleotides, mRNA and protein therapeutics.
$289,850 - $392,150ExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Provide assistance in the execution of experiments and analytical procedures including: molecular biology, DNA cloning, cell culture, protein production, ELISA, Biolayer interferometry and HPLC.
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Characterize recombinant antibodies/proteins using SDS-PAGE, protein quantitation, HPLC, ELISA and other biochemical assays to determine protein purity and function. Bachelor's or Master's degree in Biochemistry, Chemistry, or a related field with a minimum of 3 years of protein purification experience.
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Work closely with synthetic chemistry, toxicology, ADME groups, analytical teams and device teams to develop the drug product that is stable and patient centric. Represent Drug Product team in CM&C teams and support the formulation and process development of protein, peptide, oligonucleotides and other novel modalities in clinical development.
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Experience with protein purification using an AKTA system and protein characterization using HPLC (analytical SEC), SDS-PAGE, and mass spectrometry. Perform standard protein QC, including SDS-PAGE, analytical SEC, and intact mass spectrometry.
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In this role, you will play a pivotal part in QPS’ continued expansion of our bioanalytical capabilities to support small molecule, antibody drug conjugate, protein, and gene therapy drug development.
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Requirements Experience with human cell culture, protein assays (plate based ELISAs, immunostaining-based assays, fluorescent microscopy), quantitative PCR, RNA and protein isolation and quantification methods required.
$150,000 - $190,000 a yearFull-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Experience with protein purification and in vitro analytical techniques for protein characterization (e.g., SEC, MS) Understanding of protein structure and function. As a scientist you will be responsible for the design and execution of in vitro display technologies for lead antibody identification/optimization and collaborating to generate stage-gate appropriate in vitro / cellular / in vivo data packages supporting PoC. The candidate will leverage expertise to develop and implement research to gain a mechanistic understanding of the molecular pharmacology of therapeutic molecules.
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Clinical Bioinformatics & Exploratory Analytics, as a part of the Clinical Data Science organization, supports drug development and other business use cases across Gilead by providing data analytical, engineering, and visualization solutions to enable scientific and other business exploration and decision-making, including statistical analysis, genomic data processing and analysis, general machine learning and deep learning, and other technical infrastructure.
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Display competency with process equipment and automated control systems Knowledge of biotech manufacturing process and equipment including cell culture, depth and tangential flow filtration, column chromatography, protein purification and formulation and/or buffer and media operations.
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Solid technical ability and hands-on experience with molecular biology, ribonucleic acid ( RNA) and protein analysis, analytical gel electrophoresis, quantitative polymerase chain reaction (qPCR),enzyme-linked immunosorbent assay (ELISA), western blot, flow cytometry, quantitative reverse transaction (qRT-PCR), microscopy, histology, and cell culture.
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