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Strong grasp of biochemical methods for protein purification, including affinity, IEX, SEC, HIC, and MMC. Sr. Scientist/Principal Scientist– Protein Biochemist. · Proficiency in structural biology and computational tools to aid in designing of protein constructs.
Full-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Hands-on experience in protein purification using HIC, IEX, SEC, and affinity chromatography, preferably with GE AKTA FPLC. Experience in protein engineering and production would be advantageous.
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Follow established protocols and carry out small- and large-scale protein purifications (affinity, ion exchange, and size exclusion chromatography) Miscellaneous work related to protein chemistry.
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Knowledge of protein biochemistry and associated characterization techniques (UV-Visible spectroscopy, HPLC-SEC/IEX/bio-affinity methods, rheology) is required; Hands-on experience with tangential flow filtration (TFF) of protein solutions is desirable.
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Transfers and Introduces technology of cell therapy-based and therapeutic protein processes into manufacturing collaborating extensively with Scorpion’s Process Development group to develop cGMP-compliant, clinical-scale production processes.
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Responsibilities: Establish and maintain a state of the art analytical development laboratory that will allow for rapid process development support and development of control system and characterization methods to determine potency by ELISA, as well as process related impurities including host cell protein, host cell DNA, affinity resin materials, etc.
$191,335 - $247,610 a yearFull-timeExpandApply NowActive JobUpdated 5 days ago - UpvoteDownvoteShare Job
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Experience with protein purification techniques (Affinity, IEX, HIC, SEC, etc), instrumentation (FPLC), software (Unicorn, ChromLab) Familiarity with protein analytical techniques (HPLC, MCE, etc) and analysis software (Chromeleon, LabChip, Empower, etc.
$23 - $24 an hourExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Perform protein purification using AKTA systems and various chromatographic techniques (affinity, ion exchange, size exclusion chromatography, etc.) You will utilize your expertise in protein purification techniques to purify antibodies and other proteins of interest, ensuring high yield and purity.
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Apply specific expertise in protein purification techniques, including affinity-tag, ion exchange, and size-exclusion chromatography. Specific expertise in protein purification techniques, including affinity-tag, ion exchange, and size-exclusion chromatography is strongly preferred.
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The major role is to develop advanced proteomics approaches for affinity-enriched nanoLC-MS/MS. You will be a key contributor to enable high-resolution de novo spatial proteomics. Excellent knowledge and practical experience with preparation techniques of proteomics sample, nanoLC-MS/MS workflows, data processing for large-scale protein characterization, and quantification applications.
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Experience with FPLC systems and with affinity, IEX and SEC protein purification methods is desirable. Characterize recombinant antibodies/proteins using SDS-PAGE, protein quantitation, HPLC, ELISA and other biochemical assays to determine protein purity and function.
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Approaches include gain- and loss-of-function genetics coupled with phenotypic analysis, phosphoproteomics, enzymatic analysis of kinase and phosphatase activities, and identification of protein interaction networks using yeast 2-hybrid and affinity purification-mass spectrometry approaches.
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The Specialist at the Associate or Full rank will support a number of local, national and international collaborative research projects involving the discovery of new proteins using a number of methods based on affinity methods for sample isolations, chromatography for separation of complex mixtures and mass spectrometry-based peptide sequence analyses for identification of both knowns and unknowns.
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Perform expression and purification of recombinant viral protein and human monoclonal antibodies and fragments ( E.coli, insect cells, mammalian cells) via affinity chromatography and FPLC-based size exclusion and ion exchange.
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Molecular Biology, such as PCR and gene cloning; Biochemistry, such as protein purification and affinity measurement; Cell Biology, such as cell fusion assays; Virology, such as viral neutralization assays (non-pathogenic); Structural Biology, such as X-ray crystallography and Electron Microscopy analysis; and Animal Study, such as mouse immunization.
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protein affinity jobs
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