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Lead, design and optimize research with aims of exploring next generation cell therapies using CRISPR based. The candidate will be an integral part of our R&D team, whose aim is to research, develop and advance the next generation cell therapies using cutting edge genome editing technologies and create innovative novel CAR designs for a wide range of human indications.
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This specialist will assist with flow cytometry, clinical and preclinical specimen preparation for cytometry, cell sorting, and 10X RNA seq, cell culture, preclinical mouse research (including surgical procedures), molecular biology and immunology in a new translational cancer research laboratory at UCSF with a focus on next generation cancer immunotherapeutics.
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Proficiency in molecular biology techniques such as DNA/RNA extraction, gene expression analysis (PCR/RT-PCR), western blotting, ELISA, and Next Generation Sequencing. If your qualifications align with the requirements of this role, we invite you to apply and take the next step in your career journey.
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In this role, you will work as part of the QC team and responsible for effectively managing the QC process of Takara’s Reproductive Health Technology (RHT) and Next Generation Sequencing (NGS) product portfolio.
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Poseida’s proprietary gene-editing technologies, the Cas-CLOVER and Super piggyBac platforms, are the foundation for our next generation Gene Therapy products. Based in San Diego, Poseida Therapeutics is a clinical-stage biopharmaceutical company utilizing our unique and proprietary genetic engineering platform technologies to create next-generation cell and gene therapies with the capacity to cure.
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Experience with next-generation sequencing (DNA & RNA), and large-scale pipette projects/testing. Experience with next-generation sequencing (DNA & RNA), and large-scale pipette projects/testing.
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You will have deep technical savvy, a strategic mindset, and clear, forwarding-thinking leadership in AI/ML, computational biology and molecular biology, with a proven track record of leveraging computational tools to drive innovation in biotechnology, particularly in the realm of molecular biology, genome/transcriptome-wide analysis, single cell analytics, molecular design and structure, next generation sequencing, to predict RNA structure, regulation, and splicing.
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Perform analysis of in vitro and in vivo editing outcomes using next-generation sequencing, quantitative PCR, and RNA sequencing. About Metagenomi Metagenomi is a gene editing company committed to developing potentially curative therapeutics by leveraging a proprietary toolbox of next-generation gene editing systems to accurately edit DNA where current technologies cannot.
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Responsible for transfection, lentiviral transduction, flow cytometry, cell sorting, next generation sequencing, & performing CRISPR screens in neuronal & glial cell types. Utilize molecular analytic methods including DNA/RNA isolation, PCR/qPCR, molecular cloning, genotyping, &/or western blot.
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Preferred: Specific experience in RNA/DNA purification and analysis, next-generation sequencing, biochemistry, or cellular imaging. Job Description :The Senior Research Assistant will independently maintain cell lines and reagents, perform transfections, PCR, western blotting, and extractions of RNA, DNA and protein.
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Perform research in next generation sequencing (NGS) including Illumina (iSeq, MiniSeq, MiSeq, NextSeq 2000 and NovaSeq 6000), and Oxford Nanopore Technologies (MinION and GridION). Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays.
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We fulfill our mission every day by educating and preparing the next generation of scientists, physicians, medical researchers and educators for 21st century careers, and by increasing public understanding of biology and encouraging scientific discovery among K12 teachers and school children.
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We offer great opportunities to learn new techniques in next generation sequencing, stem cell biology, and mitochondrial metabolism. Performs research with molecular/cell biological and histological techniques including PCR, DNA and RNA extraction, immunostaining, Western-blotting, cell culture, and preparing cryosections.
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This individual will work in close collaboration with scientists in Translational Research, Discovery, Early and Late Clinical, Discovery Sciences, Data Sciences, and Quantitative Sciences to analyze, integrate, and interpret high dimensional data from a variety of platforms including bulk and single cell DNA/RNA/TCR next-generation sequencing, gene expression microarrays, flow/CyTOF, cytokines / protein expression, proteomics, and gene expression and genotyping arrays.
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License and experience with Next Generation sequencing and Virology. Experience in complex molecular diagnostic techniques, including hands-on experience with Next-Generation Sequencing assays and review of metagenomic data analysis.
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