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Key Responsibilities: 70% - Mammalian cell culture; RNA and DNA isolation; qRT-PCR, shRNA techniques; protein isolation and immunoprecipitation-western blotting; immunohistochemistry; in situ hybridization; fresh frozen and formalin-fixed paraffin-embedded tissue preparation and sectioning; and other histological/molecular/cellular biology techniques required for the research projects.
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Performs techniques such as PCR, immunohistochemistry, electrophoresis, protein purification and others. Performs techniques such as PCR, immunohistochemistry, electrophoresis, protein purification and others.
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Experience and knowledge with neural cell culture, protein, DNA and RNA purification, animal surgery and mouse colony management. Experience with animal studies, immunohistochemistry, or RNseq/ChIP-seq analyses.
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The RA I will be able to perform routine cellular, micro-and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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Performs experimental procedures of molecular and cellular biology involving tissue culture, RNA, DNA and protein processing, PCR, western blot, flow cytometry and immunohistochemistry, etc.
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Performs routine laboratory tests (i.e.: cell culture, immunohistochemistry, molecular assays, Western blotting, protein purification, ELISA, etc) and synthesizes and interprets results.
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Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry. Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.
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Modern research laboratories are equipped for tissue culture, real-time PCR, techniques for advanced protein detection, laser confocal microscopy, flow cytometry and molecular biology. Special diagnostic testing includes immunohistochemistry, in situ hybridization, advanced molecular diagnostics, electron microscopy, and whole slide imaging.
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Designs and executes scientific experiments which frequently utilize methods such as protein detection and quantification, enzymatic and other cell-based test methods, immunohistochemistry, microscopic image analyses, and biomaterials testing.
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Conduct manual immunohistochemistry (IHC) assays to detect specific protein markers in tissue samples. Histotechnician (HT)/Histotechnologist (HTL) certification from American Society for Clinical Pathology (ASCP.
$70,000 - $92,000 a yearFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Cell culture maintenance under faculty supervision: Generation, propagation and maintenance of sterile cell cultures and intestinal organoids, performing basic in vitro experiments, including but not limited to harvesting, splitting, and freezing cells, drug delivery to cultures, protein, and RNA extractions.
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Experience with flow cytometry, mass cytometry (CyTOF), and/or immunohistochemistry. Performing flow cytometry and immunohistochemistry experiments. Performing a range of molecular biology techniques, including molecular cloning, DNA and RNA isolation, and protein expression analysis.
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Immunohistochemistry and microscopy studies on brain tissue and cell culture systems. The ideal candidate will have deep expertise in molecular biology and biochemistry, Parkinson's disease and neurodegenerative disorders, as well as pathways involved in protein degradation and proteostasis.
$64,480 - $66,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Experience with BCA protein quantification, Western blot analysis and immunohistochemistry (IHC) staining; Performing gene cloning, RNA extraction, qRT-PCR, DNA gel electrophoresis and purification, western-blot analysis, immunohistochemistry, etc.
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Trained to perform genetic, biochemical, molecular, and cellular techniques in experimental disease model systems including but not limited to: DNA/RNA/protein extraction and quantification, SDS-PAGE/immunoblot, PCR, real-time qPCR, immunofluorescent and immunohistochemistry, biochemical assays, cell culture, rodent colony management, transgenic rodent models, rodent exposures, and mammalian tissue collection and processing.
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