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The Research Assistant I will be able to perform routine cellular, micro- and molecular biology procedures including, but not limited to Western, Northern blot, DNA/RNA, PCR, protein extraction, cell culture, Immunohistochemistry, and staining.
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This includes neuroimaging, behavioral tests, stereotaxic injection, tissue and cell culturing, tissue collection and sectioning, immunohistochemistry, PCR, DNA and RNA extractions and genotyping.
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Performs experimental procedures of molecular and cellular biology involving tissue culture, RNA, DNA and protein processing, PCR, western blot, flow cytometry and immunohistochemistry, etc.
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Skills: Small animal surgery, mouse colony management, genotyping, DNA and RNA extraction, PCR, cloning, tissue cryosection, immunohistochemistry, and lab organization management. Skills: Small animal surgery, mouse colony management, genotyping, DNA and RNA extraction, PCR, cloning, tissue cryosection, and immunohistochemistry.
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The incumbent will perform tibial fractures of mice with various genotypes, evaluate the fractured bones at different times following fracture using methods including histomorphometry, immunohistochemistry, quantitative computed tomography, lineage tracing, cell culture, and genome analysis analyzing the effect of abaloparatide on these processes.
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Performs routine and non-routine laboratory tests which may include DNA sequencing, gene cloning, in situ hybridization, immunohistochemistry, northern blotting; synthesizes and interprets results.
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Designs and executes scientific experiments which frequently utilize methods such as protein detection and quantification, enzymatic and other cell-based test methods, immunohistochemistry, microscopic image analyses, and biomaterials testing.
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Other responsibilities include data analysis and maintenance of tissue, DNA, and other experimental samples. Experience in veterinary or animal research support functions such as anesthesia and animal surgical support preferred.
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Performing gene cloning, RNA extraction, qRT-PCR, DNA gel electrophoresis and purification, western-blot analysis, immunohistochemistry, etc. Biochemistry and immunology including immunohistochemistry (antibody) staining of cells and tissues, ELISA, and immunofluorescence assays.
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Trained to perform genetic, biochemical, molecular, and cellular techniques in experimental disease model systems including but not limited to: DNA/RNA/protein extraction and quantification, SDS-PAGE/immunoblot, PCR, real-time qPCR, immunofluorescent and immunohistochemistry, biochemical assays, cell culture, rodent colony management, transgenic rodent models, rodent exposures, and mammalian tissue collection and processing.
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Skill/knowledge of immunohistochemistry, and/or Leica BOND RXm Autostainer. DNA, RNA, Protein isolation, purification and expression Southern, Northern, Western blotting. The Northwestern campus sits on the traditional homelands of the people of the Council of Three Fires, the Ojibwe, Potawatomi, and Odawa as well as the Menominee, Miami and Ho-Chunk nations.
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Performs RNA/DNA/Protein Isolation, RT-PCR, ELISA/Western Blot, Immunohistochemistry & Immunofluorescence. The candidate should be proficient in RNA/DNA/protein isolation, RT-PCR, western blot/ELISA.
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Assays include immunohistochemistry, flow cytometry, immunofluorescence, DNA damage assays, RNA and DNA analysis, and immune cell functional assays. Experience with DNA damage assays such as COMET and TUNEL. Proficiency with immunological techniques including flow cytometry, ELISA, ELISPOT, RNA and DNA isolation is a plus.
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Preparation of brain tissue and performance of immunohistochemistry, Western blotting, RNA extraction, DNA synthesis and PCR as needed. Demonstrated knowledge in cell culture techniques, DNA extractions, and PCR.
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Experience with general laboratory techniques, especially basic molecular and cellular biology techniques (including but not limited to mammalian cell culture, DNA and RNA isolation, PCR, immunohistochemistry, DNA assembly, bacterial transformation.
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