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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR , Flow Cytometry ) drug screening as well as understanding molecular pathways (Proliferation, Apoptosis, Flow.
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Performs various tasks including engineering targeting constructs, gene knockdown, genotyping by PCR and Southern blot analysis, studying gene expression and protein synthesis by qRT-PCR, western blot, and flow cytometry.
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The incumbent will be responsible for drug screening, cell-based assay development, and biomarker discovery. transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and.
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Expertise and hands-on experience in immunological assays, including IHC, ChIP, ELISA, IF/ICC, flow cytometry, western blot, etc. Expertise and hands-on experience in immunological assays, including IHC, ChIP, ELISA, IF/ICC, flow cytometry, western blot, etc.
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Experience with immunohistochemistry and flow cytometry. The ARS/AsRS is also responsible for conducting in vitro and in vivo experiments to evaluate the efficacy and safety of novel radiopharmaceuticals in non-clinical models.
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The postdoctoral scholar will apply complementary approaches including isolation of autophagosomes, autolysosomes and late endosomes, flow cytometry and super-resolution microscopy to determine the mechanisms of sequestration of lipid cargo by autophagy.
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Core resources include flow cytometry, confocal microscopy, state-of-the-art proteomics and genomics centers, extensive biosafety level three facilities, and newly renovated animal facilities.
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The MPIM Department nurtures a highly interactive group of faculty and houses several state-of-the-art core facilities including live animal imaging, microscopy, flow cytometry, BSL-2,-3 labs, and excellent AAALAC-accredited conventional, and ABSL-2, ABSL-3 animal facilities.
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Numerous opportunities exist for collaborations in basic and clinical sciences as well as interdisciplinary programs with the School of Veterinary Medicine and other components of Texas A&M University and the Health Science Center campuses, including the Institute of Biosciences and Technology in Houston (Qualifications Candidates must have earned a PhD, MD, DVM or equivalent in their research discipline.
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Position Summary The Senior Associate, Analytical Development will be responsible for the execution of routine testing, including but not limited to flow cytometry assay, cell-based cytotoxicity assay, or PCR-based assay, to characterize or release engineered TCR-T cell drug product.
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The primary responsibilities of the RAs will include isolation and culture of primary human immune cells, functional analysis of engineered cells via high throughput flow cytometry, assistance with genomic techniques such as single-cell RNA sequencing and CRISPR gene editing, among others.
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Solid understanding in small animal surgery and/or flow-cytometry is highly desired. We are seeking an experienced Scientist to join the Immuno-Oncology group to support our efforts on developing strategies to overcome immune-checkpoint inhibitor therapy resistance in liver cancers including primary hepatocelluar carcinoma (HCC) and tumors from other organs metastasized to livers.
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Experience with cell-based assays, immunofluorescent microscopy, qPCR, flow cytometry, single-cell genomics, RNAseq, cell culture, organoid culture, Illumina NextSeq 550, DNA methylation sequencing, ddPCR, Luminex, clinical chemistry instrumentation, Kryptor, Access 2, robotic liquid handling (e.g. Tecan), Vortex, and DepArray.
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You’ll be responsible for supporting the ramp up of new QC labs, performing key QC lab techniques including ELISA assays and flow cytometry platforms. Hands-on experience working with flow cytometry and cell counting platforms such as FACS.
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Perform viral immunology techniques such as immune cell isolation & culturing, primary cell transduction using LV/AAV vectors, multi-parametric flow cytometry, and cell-based functional assays (e.g., virus/vector titration, ELISA, and cytotoxicity assays.
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