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Research in the Dovat lab uses molecular and cellular biology techniques including qRT-PCR, quantitative chromatin immunoprecipitation (qChIP), ChIP-seq, ATAC-seq, CUT & TAG, retroviral and lentiviral production, transfection, luciferase reporter assay, tissue culture, cloning, DNA and RNA isolation, Western blot, protein purification, flow cytometry, as well as isolation of hematopoietic cells from mouse bone marrow and spleen.
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We embrace individual uniqueness, foster a culture of inclusive excellence that supports both broad and specific diversity initiatives, leverage the educational and institutional benefits of diversity, and engage all individuals to help them thrive.
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Technical Computer Software literacy, including GraphPad Prism, Softmax, and Flow Cytometry based applications (CellQuest, FACSDiva, and FlowJo). This position requires basic knowledge of cellular immunology and experience with tissue culture.
$27.88 - $37.5 an hourExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Preferred qualifications: Familiar with techniques such as multi-color flow cytometry and/or mass cytometry, sterile technique for cell culture with BSL-2+ practices, qRT-PCR, sterile technique for bacterial culture, and animal dissection and tissue processing.
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Perform cell biology and molecular biology including primary and tumor cell culture, lentiviral transductions, RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and ELISA. Some training may be provided.
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Responsibilities will include assisting in the in vitro characterization of the pharmacology of the radiopharmaceuticals by performing techniques like tissue culture, protein expression and purification, immunoblot, flow cytometry, biolayer interferometry, qPCR. Moreover, this role will require direct animal handling, which will include establishing and maintaining an animal colony of mouse models of human cancer, and small animal imaging and treatment studies.
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Specific duties include tissue cell culture, flow cytometry, PCR, western blots, immunofluorescence, animal work, preparation of data for publication and grant proposals, ordering of supplies and equipment, upkeep of equipment and general lab maintenance.
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The methods used in our laboratory include tissue culture, exposure of cells to cigarette smoke and e-cigarettes, isolation of cells from murine and human tissues, transfections, RNA and protein isolation, quantitative PCR, Western blotting, histology, immunofluorescence, fluorescence microscopy, flow cytometry, and mouse models of lung disease.
$19 - $21 an hourFull-timeExpandApply NowActive JobUpdated Yesterday - UpvoteDownvoteShare Job
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Perform tissue culture work with primary cells, cell staining, flow cytometry. Perform PCR and gel electrophoresis for tissue sub-typing. Perform animal manipulations independently – injections (retro-orbital, intra-peritoneal, subcutaneous), retro-orbital bleeding, euthanasia, tissue harvesting, biopsies and record detailed observations into a database.
$28.04 an hourFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Good written/oral communication skills in English, and experience in cellular and molecular biology techniques (e.g., flow cytometry, RNA isolation, protein analysis methods), rodent models and cell culture in vitro models are highly desirable.
$50,000 - $60,000 a yearFull-timeExpandApply NowActive JobUpdated 2 days ago - UpvoteDownvoteShare Job
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Preferred Laboratory Skillsets: Aseptic technique, cell and tissue culture, cell-based assays for characterizing cell and gene therapy products, ELISA, dPCR, TCID50 assays, NGS experience, flow cytometry, sterility testing, bioburden testing, etc.
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Proficient in laboratory bench skills such as Tissue culture, ELISAs, Western blots, qPCR, Flow cytometry, and histology. functional assays using, primary cells, human and rodent tissue samples and human matrices with readouts such as multi-parametric flow cytometry, multiplex ELISA, and application of these techniques to pharmacological studies.
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Extensive knowledge in various techniques including but not limited to cell culture of mammalian cell lines and primary tissue samples, cell-based assay development, western blotting, MSD, gene editing and cell line generation, qPCR, Nanostring/RNASeq, siRNA/shRNA knockdown, immunofluorescence, flow cytometry and other basic molecular and cell biology skills.
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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR , Flow Cytometry ) transductions , RNA and protein preparation from cells and tissue, RT-PCR, western blotting, and.
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Experience in immunological methods such as ELISA, antibody characterization, and western blot; proficiency in PCR amplification, cloning, protein expression and purification, and tissue culture techniques.
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