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Proficiently perform quality control assays and exploratory bioanalytical assays to phenotypically and functionally characterize cells following expansion, including flow cytometry, fluorescence microscopy, qPCR, and cellular functional assays.
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Plan, coordinate and execute analytical testing of molecular assays such as qPCR/ddPCR and flow cytometry-based assays. Experience with relevant technologies with an emphasis on potency and functional assays, cell culture (e.g. aseptic techniques, cell line expansion), flow cytometry-based assays and ddPCR.
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Specific skills required for Molecular Pathology, Flow Cytometry and Point of Care job postings. Specific skills required for Molecular Pathology, Flow Cytometry and Point of Care job postings.
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Flow cytometry, qPCR/ddPCR, cell-based potency and/or ELISA experienceExperience with method qualification and/or validation. BS of MS degree in Biology, Biochemistry, Molecular Biology or other relevant field.
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Experience in Flow Cytometry. Masters or Ph. D. Students in Immunology, Molecular Biological Sciences, Cancer Biology, or related fields are also encouraged to apply. Must be enrolled in a university program pursuing a Bachelor’s in Immunology, Molecular Biological Sciences, Cancer Biology, or reds.
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Extensive experience in flow cytometry, as well as molecular and cellular biology; Collaborate with CRO and academic partners; employ cutting-edge in vitro and in vivo animal and human models to advance drug discovery and development programs up to and including IND filing.
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Hospital based laboratory has state of the art in-house flow cytometry, cytogenetics/FISH and molecular lab (including NGS) thereby providing comprehensive hematopathology services.
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Demonstrate proficiency with sterile cell culture, cell-based assays (CTG, apoptosis) and molecular biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry.
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The Assistant Director keeps abreast of the most advanced technologies available in genomics, flow cytometry, and biological imaging and supports strategic, administrative, and operational standard operating procedure development and refinement to make these tools available to UO investigators as technology evolves.
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Experience with tissue culture, flow cytometry, standard molecular, cell biology and biochemistrytechniques highly desireable. Our laboratory uses genomics and a wide range of molecular and cellular biological techniques to elucidate the molecular basis of cancer and hematologic disorders, with the aim of translating these insights into novel therapies for patients with cancer.
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Molecular biology, mammalian cell culture, research, cell culture, tissue culture, cell line, Assay, assay development, next generation sequencing, sequencing, crispr, protein engineering, python, rna engineering, flow cytometry, pipetting, liquid handling, sop.
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Experience using software for flow cytometry analysis, electronic lab notebook and data visualization. · Technical expertise in at least two of the following areas: cell based functional assays, image acquisition/analysis, molecular assays (NGS, RT-PCR, dd-PCR.
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Experiences in cell and molecular biology and immunological techniques, including cell culture, Western immunoblotting, QRT-PCR, gene transfection and cloning, RNA interference and DNA sequencing, flow cytometry-based assessment of immune system mechanisms that regulate cancer initiation, promotion and progression, the role of the tumor microenvironment in cancer immunity and novel approaches to cancer immunotherapy.
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One year recent experience in Flow Cytometry analysis such as leukemia / lymphoma phenotyping, paroxysmal nocturnal hemoglobinuria, T cell subset enumeration, DNA cell cycle analysis and cytochemical staining.
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Demonstrated ability with cell-based assays and molecular biology techniques including, but not limited to, immunoassays (ELISA, Western Blots), PCR, qPCR, and flow cytometry. + Utilizes various biochemical, molecular, cellular, and functional techniques to develop and validate complex assays using mammalian cells, tissues, and recombinant proteins to interrogate biological pathways of interest and support emerging biological concepts.
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