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Experience with wet lab approaches, including biological sample processing, ELISA, qPCR, in vitro cellular and molecular approaches, including cell culture, protein analysis with BCA and Western blot, immunostaining, microscope imaging, and flow cytometry.
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MS (2-4 years experience) or BS (6+ years experience) in immunology, biochemistry, molecular biology, bioengineering, or related field. Flow cytometry (Cell binding, ligand blocking, etc.
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A strong background in Flow Cytometry, Immunohematology, Immunology, and/or Molecular Biology techniques is preferred. Testing performed: HLA Flow PRA screen; Luminex Single Antigen (LSA) HLA Class I & II; Flow cross-matching; ELISA; cell isolation and preservation from peripheral blood and donor tissue specimen; DNA extraction, purification, and quantitation; various molecular typing techniques to include Next-generation sequencing (NGS), RT-PCR, and low to intermediate resolution SSO/SSP typing.
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Expertise in mammalian cell culture using aseptic tissue culture techniques and cell-based assays to evaluate viability (siRNA/shRNA, small molecule compound screening), protein level analysis (western blot), gene expression analysis (qRT-PCR) and other functional/molecular biology assays.
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She/he will perform advance cellular and molecular biology techniques under the supervision of the lab manager and PI. For a level C position, She/He must work independently performing several techniques including basic cell culture, cell transfection, lentiviral particles preparation, flow cytometry, ELISA, Immunohistochemistry and animal models.
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Technical expertise in molecular biology/antibody techniques, instrumentation, and assays performed. Provides support by providing flow cytometry expertise to experimental strategy, problem characterization, data acquisition and analysis.
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Learn how to assess various assay readouts through different techniques (flow cytometry, Incucyte, MSD, ELISA, microscopy) Learn methods for assessing protein levels in various bioassays from in vitro cultures (western blots [JESS], flow cytometry.
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Biology techniques (Protein, DNA and RNA isolations, Western blot, qRT-PCR, Flow Cytometry) Work at the bench to develop and execute established and novel molecular and cell-based assays for drug screening as well as understanding molecular pathways (Proliferation, Apoptosis, Flow Cytometry etc.
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Experience working with flow cytometry and standard molecular biology techniques. Required technical skills include: RNA and DNA extraction, tissue culture, and flow cytometry techniques.
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3+ years working as an MT/CLS : Cellular Therapy Laboratory, Blood Bank/Flow Cytometry/Molecular Oncology experience is preferred. ASCP Certification (or equivalent) in Blood Bank, Molecular Biology, Medical Laboratory Technician, Medical Laboratory Science, or Clinical Laboratory Science, Bachelors Degree.
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Candidate would be tasked with a variety of molecular biology, biochemistry, and cell culture experiments aimed at deducing the structure of antibody-epitope pairs. Experience with flow cytometry.
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Experience in cell culture and cell-based assay development, T-cell biology, and flow cytometry is helpful but not required. This position offers exposure to additional areas of this cutting-edge program, such as cell biology and molecular biology, immunology, oncology, autoimmunity, and cell therapy.
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Independently perform cellular and molecular biology assays including: flow cytometry, ELISA, PCR, and immunocytochemistry. Bachelor of Science degree in Biology, Molecular Biology, Physiology or a related discipline.
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Laboratory experience using clinical samples, flow cytometry, and/or genomics. Laboratory experience using cell culture and molecular biology techniques. The ideal candidate should have research experience with patient tumor specimens, mammalian cell culture, and standard molecular biology techniques.
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Expertise with laboratory methods including flow cytometry, qPCR, RNA/DNA isolation. Experience with mammalian cell culture methods and molecular biology skills. Expertise with laboratory methods including flow cytometry, qPCR, RNA/DNA isolation.
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