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Deep technical understanding of analytical method development for key assays (Flow Cytometry, CCV, ddPCR, qPCR, ELISA, HPLC, cell-based potency) and associated requirements (validation, stability programs, comparability assessments, etc.
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Proven experience in laboratory cellular biology techniques applied to development of physiologically relevant cellular assays to evaluate biological mechanisms (including but not restricted to primary cell culture, flow cytometry and ELISAs.
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Experience with flow cytometry, PCR and protein detection preferred. B.S. in a biological science, chemical engineering or other relevant discipline with 5+ years of relevant experience in cell therapy development.
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Candidates will perform molecular biology techniques, particularly cloning targeting constructs and CRISPR plasmids, assist in culturing and differentiating human stem cells, and analyze RNA and protein expression via qPCR analysis, immunofluorescence, flow cytometry and Western blot.
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Our core technology, Ghost Cytometry (GC), combines flow cytometry, high-speed imaging, and machine learning to enable the characterization and isolation of cells based on cellular morphology.
$65,000 - $80,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Experience with flow cytometry, bioassays, and molecular biological assays. Experienced with analytical test methods used for cell/gene therapy (e.g. flow cytometry, cell-based assays, PCR, and ELISA test methods.
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In-depth knowledge of relevant cell therapy analytics including Flow Cytometry, qPCR, ddPCR/dPCR, cell-based assays, sterility, and biological potency. In-depth knowledge of relevant cell therapy analytics including Flow Cytometry, qPCR, ddPCR/dPCR, cell-based assays, sterility, and biological potency.
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Work products and documents related to preparing blood by whole lysis or density sedimentation methods; prepare cell samples for flow cytometry; extract DNA and RNA from human and murine blood and marrow; perform molecular and cell biologic protocols; maintain bacterial and tissue cultures.
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Experience with advanced cell-based immunoassays such as cytotoxicity assays, live cell imaging techniques, cytokine measurements, flow cytometry. Expertise in the design and execution of cellular experiments to determine the effects of biotherapeutics on biological systems.
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Basic molecular biological and flow cytometry skills are preferred. Bachelor’s degree in Biomedical Engineering, Biological Sciences or related field. Basic molecular biological and flow cytometry skills are preferred.
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Performs specimen receipt, accessioning for all samples received in the Flow Cytometry department. Previous Flow Cytometry sample processing experience. Flow Cytometry-Ref Lab.
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Experience with laboratory analytical techniques, such as flow cytometry, microfluidic systems, fluorescence microscopy, HPLC. NanoFCM produces analytical devices for research & development based on nano-flow cytometry (nFCM.
$55,000 - $80,000 a yearFull-timeExpandApply NowActive JobUpdated Today - UpvoteDownvoteShare Job
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Experience in biological / immune assays and flow cytometry and analysis (DIVA, FLOWJO) is preferred. Details can be immunology/cell biology related various biological assays, analyzing patients’ samples and tumor-specific effector cells function, molecular biology related to cloning, DNA/RNA extract, and transduction to lentivirus or retrovirus vectors, and assisting with other lab tasks as needed.
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Primary duties include 3-4 steps synthesis of novel lipids, lipid-nanoparticle formulations and characterization, cell culture, blood collection, tissue harvesting, RT-qPCR, ELISA, and flow-cytometry; imaging techniques such as bioluminescence and fluorescence; and general lab support, e.g., ordering and maintaining solvents/chemicals stock.
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Analysis may include immunological procedures including Enzyme Linked immunoassay (ELISA), Western blot, Flow Cytometry and RNA/DNA sequencing assays. In this role, the Laboratory Technician shall aid in the execution of scientific studies including extraction of nucleic acids from different biological matrices, quantification and quality of RNA and DNA, PCR amplification, sequencing library preparation and quantification, high-throughput screening assays and/or high-throughput sequencing on infectious agents.
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