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A strong background in cell culture and multi-color flow cytometry is preferred. Design and execute project plans involving immune cell profiling, cell processing from xenograft models, cytotoxicity, proliferation, activation, cell viability, and survival assays with a strong focus on flow cytometry.
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Develop and conduct screening tests including ELISA, flow cytometry, and cell-based assays such as opsonophagocytosis and complement activation for antibody characterization. Experience with microbiology of Gram-negative species, antibacterial development, genetics and molecular cloning to determine and develop target antigens, eukaryotic cell culture, expression vector design, transient transfection, recombinant antibody testing and development, protein expression.
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Extensive experience in flow cytometry, as well as molecular and cellular biology; Develop and execute efficient strategies for target identification, prioritization and validation; review, propose, initiate, and implement new pre-clinical therapeutic antibody drug discovery and development programs in the field of Immunology and Inflammation.
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Learn additional methods as needed including, but not limited to; ELISA, flow cytometry, laser capture dissection, and qRT-PCR. This may include documentation of having two (2) MMR vaccines; two (2) Varicella vaccines; or antibody status to these diseases from laboratory testing.
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First-hand knowledge of and experience with assays relevant for cell therapies including flow cytometry, IHC, qPCR, anti-therapeutic antibody assessment, T-cell antigen specific response assays (ELISPOT, flow based, tetramer, etc.
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Participate in the construction and screening of immune-based libraries through immunization, antibody display, and flow cytometry. Visterra, Inc., is seeking a highly motivated Research Associate with research experience in biochemistry, cellular and/or molecular biology to help discover and develop biologic therapeutics, as well as expand platform technologies that support our antibody-based drug discovery programs.
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Running immunoassays, such as ELISA, and using flow cytometry, in order to determine immune responses. Experience in cell culture, immunoassays, and flow cytometry is preferred.
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Experience in multi-color flow cytometry. The position will report directly to the CEO of the company and work as a lead member of the antibody discovery and preclinical development team.
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Ability to design antibody panels for multi-color flow cytometry. Ability to design antibody panels for multi-color flow cytometry. Under the supervision of a project leader or a senior scientist, perform laboratory work to support scientific discovery programs related to skin biology.
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Our preclinical pipeline extends beyond to biologics against novel immuno-oncology targets, antibody-drug conjugates, bispecific and trispecific antibodies, and small molecules expected to reach the clinic in 6-24 months, underscoring our bench-to-bedside approach.
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Clinical consultations as well as involvement in the development of technical protocols for clinical testing including HLA typing (SSP, qPCR, NGS), crossmatching (flow cytometry), solid phase HLA antibody testing.
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Proficient in multi-parameter flow and/or spectral cytometry (antibody titration, panel design, sample acquisition, data processing) and data analysis (FACSDiva, FlowJo, GraphPad Prism, Excel.
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Flow cytometry antibody titration. + Flow Cytometer (FACS) and Genetic Analyzer maintenance. In 2020, ESFM (formerly Eurest Services) won the George Graves Award for Facility Management Achievement from the International Facilities Management Association (IFMA.
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Skills & Abilities: Extensive scientific HLA knowledge and technical skills in the following areas preferred: HLA serology and molecular genetics, including NGS, platelet serology, HLA antibody screening /identification, Flow Cytometry Crossmatch, chimerism study, etc.
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Analysis of flow cytometry results will be done using FlowJo or Cyflogic programs and involves analysis of fluorescence readouts, live/dead cells discrimination and multi color compensation.
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